Echinococcus granulosus infection reduces airway inflammation of mice likely through enhancing IL-10 and down-regulation of IL-5 and IL-17A

• Published in: Parasites & vectors Vol. 7; no. 1; p. 522
• Main Authors: Wang, Hui, Li, Jun, Pu, Hongwei, Hasan, Bilal, Ma, Jinfeng, Jones, Malcolm K, Zheng, Kan, Zhang, Xue, Ma, Haimei, McManus, Donald P, Lin, Renyong, Wen, Hao, Zhang, Wenbao
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 20.11.2014; BioMed Central Ltd; BioMed Central; BMC
• Subjects: Albumin; Allergic asthma; Allergies; Animals; Asthma; blood serum; Chinese medicine; Cystic echinococcosis; Cysts; Cytokines; dogs; echinococcosis; Echinococcosis - metabolism; Echinococcosis - parasitology; Echinococcus granulosus; enzyme-linked immunosorbent assay; Eosinophils; Epidemiology; Female; Gene Expression Regulation; goblet cells; Health aspects; histopathology; Hospitals; hyperplasia; IL-10; IL-17A; IL-5; Immune response; immunoglobulins; Infections; inflammation; Inflammation - metabolism; interleukin-10; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-17 - genetics; Interleukin-17 - metabolism; interleukin-5; Interleukin-5 - genetics; Interleukin-5 - metabolism; intermediate hosts; Laboratory animals; larvae; lungs; Mice; Mice, Inbred BALB C; mucus; ovalbumin; Ovalbumin - toxicity; Pathology; pneumonia; Public health; Respiratory Tract Infections - metabolism; reverse transcriptase polymerase chain reaction; Rodents; Specific Pathogen-Free Organisms; Studies; tapeworms; zoonoses; Queensland Australia
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-014-0522-6

BACKGROUND: Cystic echinococcosis (CE) is a near cosmopolitan zoonosis caused by the larval stage of the dog tapeworm Echinococcus granulosus. E. granulosus infection induces a polarized T-helper type 2 (Th2) systematic immune response in its intermediate hosts. However, it is not known whether the infection modulates lung inflammation by regulating local immune response. In this study, we examined the effects of E. granulosus infection on mouse ovalbumin (OVA)-induced asthma model. METHODS: BALB/c mice were intraperitoneally transplanted with 50 small E. granulosus cysts cultured in vitro. At 3 months post-inoculation, the mice were sensitized and challenged with ovalbumin (OVA). For histopathological studies, hematoxylin eosin and periodic acid schiff staining was used to examine the inflammatory cells infiltration and goblet cells hyperplasia, respectively. Cytokine levels were measured by mouse cytometric bead array (CBA) Kit and quantitative RT-PCR and other molecular biological approaches. Airway hyperresponsiveness was assessed in response to increasing doses of methacholine. Serum immunoglobulins were determined by ELISA. RESULTS: E. granulosus infection significantly increased Th2 and Treg cytokine levels in serum and lung tissues, but down-regulated the expression of IL-5 in the lungs and IL-17A in serum and lung tissues of asthmatic mice sensitized and challenged with OVA. Histological staining of lung tissues showed that E. granulosus infection significantly reduced the severity of OVA-induced airway inflammation including reduction of eosinophil cell infiltration and mucus production. The E. granulosus infection also reduced eosinophil accumulation induced by OVA in bronchoalveolar lavage fluid (BALF) and also ameliorated airway hyperresponsiveness, a hallmark symptom of asthma. CONCLUSIONS: E. granulosus infection remarkably reduces the severity of OVA-induced airway inflammation likely through enhancing IL-10 and down-regulation of IL-5 and IL-17A.