Homology modeling and functional annotation of bubaline pregnancy associated glycoprotein 2

• Published in: Journal of animal science and biotechnology Vol. 3; no. 2; pp. 68 - 76
• Main Authors: Ganguly, Bhaskar, Prasad, Shiv
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 31.05.2012; BioMed Central; Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar, PIN: 263145, India%Department of Animal Reproduction,Gynecology and Obstetrics, College of Veterinary and Animal Sciences, G. B.Pant University of Agriculture and Technology, Pantnagar, PIN: 263145, India; BioMed Central Ltd; BMC
• Subjects: Amino acids; Annotations; Apoptosis; Bubaline; complement; Fetuses; Function; genes; Glycoproteins; Homology; Homology modeling; immunity; Immunosuppression; Modelling; Molecular weight; Morphogenesis; mutants; N-糖基化; placenta; Pregnancy; Pregnancy associated glycoprotein (PAG); Proteins; Structure; Structure-function relationships; Three dimensional models; Visualization; 功能注释; 同源建模; 妊娠; 糖蛋白; 结构活性; 肽链内切酶; 蛋白质功能; Function; Pregnancy associated glycoprotein (PAG); Structure; Bubaline; Homology modeling
• ISSN: 1674-9782; 2049-1891; 2049-1891
• DOI: 10.1186/2049-1891-3-13

Background： Pregnancy associated glycoproteins form a diverse family of glycoproteins that are variably expressed at different stages of gestation. They are probably involved in immunosuppression of the dam against the fetomaternal placentome. The presence of the products of binucleate cells in maternal circulation has also been correlated with placentogenesis and placental re-modeling. The exact structure and function of the gene product is unknown due to limitations on obtaining purified pregnancy associated glycoprotein preparations.Results： Our study describes an in silico derived 3D model for bubaline pregnancy associated glycoprotein 2. Structure-activity features of the protein were characterized, and functional studies predict bubaline pregnancy associated glycoprotein 2 as an inducible, extra-cellular, non-essential, N-glycosylated, aspartic pro-endopeptidase that is involved in down-regulation of complement pathway and immunity during pregnancy. The protein is also predicted to be involved in nutritional processes, and apoptotic processes underlying fetal morphogenesis and remodeling of feto-maternal tissues.Conclusion： The structural and functional annotation of buPAG2 shall allow the designing of mutants and inhibitors for dissection of the exact physiological role of the protein.