Expression of TRAF6 and ubiquitin mRNA in skeletal muscle of gastric cancer patients

• Published in: Journal of experimental & clinical cancer research Vol. 31; no. 1; p. 81
• Main Authors: Sun, Yuan-Shui, Ye, Zai-Yuan, Qian, Zhen-Yuan, Xu, Xiao-Dong, Hu, Jun-Feng
• Format: Journal Article
• Language: English
• Published: London BioMed Central 26.09.2012; BioMed Central Ltd; BMC
• Subjects: Abdominal diseases; Adult; Aged; Aged, 80 and over; Albumin; Apoptosis; Benign; Biomedical and Life Sciences; Biomedicine; Biopsy; Blood; Body mass index; Cachexia; Cachexia - metabolism; Cachexia - pathology; Cancer; Cancer patients; Cancer Research; Care and treatment; Chronic illnesses; Comparative analysis; Female; Gastric cancer; Gene expression; Gene Expression Regulation, Neoplastic; Humans; Immunology; Male; Medical research; Medicine, Experimental; Messenger RNA; Middle Aged; Mortality; Mrna; Muscle, Skeletal - metabolism; Muscles; Muscular system; Musculoskeletal system; Oncology; proteasomes; Proteins; Proteolysis; RNA, Messenger - genetics; RNA, Messenger - metabolism; Skeletal muscle; Stomach cancer; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Studies; Surgery; TNF Receptor-Associated Factor 6 - genetics; TNF Receptor-Associated Factor 6 - metabolism; TRAF6; TRAF6 protein; Tumor necrosis factor; Tumor necrosis factor receptors; Ubiquitin; Ubiquitin - genetics; Ubiquitin - metabolism; Ubiquitin; Cachexia; Mrna; Gastric cancer; TRAF6; Skeletal muscle
• ISSN: 1756-9966; 0392-9078; 1756-9966
• DOI: 10.1186/1756-9966-31-81

Objective To investigate the prognostic significance of tumor necrosis factor receptor (TNFR),-associated factor 6 (TRAF6),-and ubiquitin in gastric cancer patients. Methods Biopsies of the rectus abdominis muscle were obtained intra operatively from 102 gastric cancer patients and 29 subjects undergoing surgery for benign abdominal diseases, and muscle TRAF6 and ubiquitin mRNA expression and proteasome proteolytic activities were assessed. Results TRAF6 was significantly upregulated in muscle of gastric cancer compared with the control muscles. TRAF6 was upregulated in 67.65% (69/102) muscle of gastric cancer. Over expression of TRAF6 in muscles of gastric cancer were associated with TNM stage, level of serum albumin and percent of weight loss. Ubiquitin was significantly upregulated in muscle of gastric cancer compared with the control muscles. Ubiquitin was upregulated in 58.82% (60/102) muscles of gastric cancer. Over expression of ubiquitin in muscles of gastric cancer were associated with TNM (Tumor-Node-Metastasis) stage and weight loss. There was significant relation between TRAF6 and ubiquitin expression. Conclusions We found a positive correlation between TRAF6 and ubiquitin expression, suggesting that TRAF6 may up regulates ubiquitin activity in cancer cachexia. While more investigations are required to understand its mechanisms of TRAF6 and ubiquitin in skeletal muscle. Correct the catabolic-anabolic imbalance is essential for the effective treatment of cancer cachexia.