Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri

• Published in: Virology journal Vol. 10; no. 1; p. 110
• Main Authors: Ren, Weicheng, Chen, Haixia, Renault, Tristan, Cai, Yuyong, Bai, Changming, Wang, Chongming, Huang, Jie
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 08.04.2013; BioMed Central Ltd; BioMed Central
• Subjects: Acute viral necrosis virus (AVNV); amino acid sequences; amino acids; Animals; Bacteriology; Base Composition; Basic Medicine; China; Chlamys; Chlamys farreri; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA - genetics; DNA viruses; DNA Viruses - genetics; DNA Viruses - isolation & purification; DNA, Viral - chemistry; DNA, Viral - genetics; Evolution; Gene Order; Genetic aspects; Genetic variation; Genetics; Genome; Genome, Viral; Genomics; Herpesvirus; Life Sciences; Medicinska grundvetenskaper; Microbiology; Molecular Sequence Data; Mollusca; mortality; necrosis; Open Reading Frames; OsHV-1; Ostreid herpesvirus 1; Pectinidae - virology; Physiological aspects; proteins; scallops; Sequence Analysis, DNA; Sequence Homology; Synteny; Viral genetics; viruses; China; France; Sweden
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-10-110

BACKGROUND: Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. RESULTS: The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb, respectively), each flanked by two inverted repeats (7.6 kb and 10.2 kb, respectively), with a third unique region (1.5 kb) situated between the two internal repeats. CONCLUSIONS: Our results indicate that AVNV is a variant of OsHV-1. The AVNV genome sequence provides information useful for understanding the evolution and divergence of OsHV-1 in marine molluscs.