Comparative Study of Two Capture Systems for the 24-kD protein of Human Immunodeficiency Virus

A comparative study between HIV-1 Antigen ELISA Test (Coulter, USA) and DAVIH-Ag P24 (DAVIH Laboratories, Cuba) was performed for the detection of HIV-1 P24 antigen. A total of 1039 serum samples of blood donors. sera were studied, in addition to 323 sera from seropositive individuals in different s...

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Published inBiotecnología aplicada Vol. 17; no. 2; pp. 102 - 104
Main Authors Izquierdo, Maricela, Silva, Eladio, Díaz, Héctor, Lubián, Ana L, Nibot, Carmen, Tabares, Dolores
Format Journal Article
LanguageSpanish
Published Cuba Elfos Scientiae 31.12.2000
Subjects
antigenemia
ELISA
HIV-1
Human immunodeficiency virus 1
marcadores de progresión
P24
P24 protein
progression markers
seroconversion
seroconversión
VIH-1
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Summary:A comparative study between HIV-1 Antigen ELISA Test (Coulter, USA) and DAVIH-Ag P24 (DAVIH Laboratories, Cuba) was performed for the detection of HIV-1 P24 antigen. A total of 1039 serum samples of blood donors. sera were studied, in addition to 323 sera from seropositive individuals in different stages of the disease. The presence of antigen was associated with the clinical group and the levels of antibodies against P24. Both systems showed a high specificity (greater than 99%) with a concordance of 99.7%. DAVIH-Ag P24 system showed a sensitivity (58/31) greater than Coulter system when it was tested in the positive population. Percents of antigenemia were greater in the AIDS group. Antibody titers against P24 were low or absent in all the sera with antigenemia to this protein. It was concluded that DAVIH-Ag P24 system can be used in the follow-up of HIV-1 infected individuals as a progression marker of the infection, and to screen P24 in blood banks.
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ISSN:0864-4551