Detection of genes for fimbrial antigens in Escherichia coli isolated from piglets with diarrhea in Camaguey

Specific DNA probes for genes encoding K88, K99, F41 and 987P fimbrial antigens were used to examine 142 Escherichia coli isolates from diarrheal cases (117 from pigs and 25 from calves). Ten isolates from pigs were adhesing gene positive with the DNA probes used: five K88+, four 987P+ and one K99+F...

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Published inBiotecnología aplicada Vol. 14; no. 4; pp. 237 - 241
Main Authors Baker, Roberto Basulto, Aguilera, Lesvia Calzada, Gutierrez, Tania Olivera, de la Fuente Garcia, Jose
Format Journal Article
LanguageEnglish
Published Cuba Elfos Scientiae 31.12.1997
Subjects
antigeno fimbrial
diarrea
diarrhea
enterotoxigenic Escherichia coli
Escherichia coli enterotoxigenica
fimbrial antigen
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Summary:Specific DNA probes for genes encoding K88, K99, F41 and 987P fimbrial antigens were used to examine 142 Escherichia coli isolates from diarrheal cases (117 from pigs and 25 from calves). Ten isolates from pigs were adhesing gene positive with the DNA probes used: five K88+, four 987P+ and one K99+F41+. All were phenotypically positive when assayed for the expression of fimbrial antigens by enzyme-linked immunoabsorbent assays. In this study, 13 % of the pigs were infected by K88+, K99+F41+ and 987P+ E. coli strains. The existence for the first time in Cuba of 987P+ and K99+F41+ native strains in pigs with diarrhea, detected with DNA probes, is reported. None of the isolates from calves resulted positive for K99 and/or F41.
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ISSN:0864-4551