Inactivation of Chikungunya virus by 1,5 iodonapthyl azide

• Published in: Virology journal Vol. 9; no. 1; p. 301
• Main Authors: Sharma, Anuj, Gupta, Paridhi, Maheshwari, Radha K
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.12.2012; BioMed Central; BMC
• Subjects: Alphavirus; Analysis; Animals; Antibodies; Antiviral agents; Arthropoda; Azides - pharmacology; Cell Line; Chikungunya virus; Chikungunya virus - drug effects; Chikungunya virus - genetics; Colleges & universities; Complications and side effects; Cricetinae; Cytopathogenic Effect, Viral - drug effects; Dosage and administration; Genetic aspects; Health sciences; Immune response; Inactivated vaccine; Infections; Iodonapthyl azide; Medical research; Physiological aspects; Polyclonal antibodies; Proteins; RNA, Viral; Short Report; Togaviridae; Ultraviolet radiation; Vaccines; Viral antibodies; Virulence (Microbiology); Virus Inactivation - drug effects; United States; India
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-9-301

Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family Togaviridae. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia. In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect and virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis. INA completely inactivated CHIKV and can further be explored for developing an inactivated-CHIKV vaccine.