Pulsed ultrasound associated with gold nanoparticle gel reduces oxidative stress parameters and expression of pro-inflammatory molecules in an animal model of muscle injury

• Published in: Journal of nanobiotechnology Vol. 10; no. 1; p. 11
• Main Authors: Victor, Eduardo G, Silveira, Paulo C L, Possato, Jonathann C, da Rosa, Guilherme L, Munari, Uillian B, de Souza, Claudio T, Pinho, Ricardo A, da Silva, Luciano, Streck, Emilio L, Paula, Marcos M S
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 12.03.2012; BioMed Central; BMC
• Subjects: Animals; anti-inflammatory; Antioxidants; Aqueous solutions; Biomedical engineering; Care and treatment; Catalase - metabolism; Disease Models, Animal; Gels - chemistry; Gels - therapeutic use; Glutathione Peroxidase - metabolism; Gold; Gold - chemistry; gold nanoparticles; Health aspects; Interleukin-1beta - metabolism; Lipid Peroxidation - drug effects; Male; Metal Nanoparticles - chemistry; Metal Nanoparticles - therapeutic use; Muscle diseases; Muscles - drug effects; Muscles - injuries; Muscular Diseases - metabolism; Muscular Diseases - therapy; Musculoskeletal system; Nanoparticles; Oxidative stress; Physiological aspects; Properties; Proteins; Rats; Rats, Wistar; reactive oxidative species; Rodents; Studies; Superoxide Dismutase - metabolism; Superoxides - metabolism; therapeutic pulsed ultrasound; Tumor Necrosis Factor-alpha - metabolism; Ultrasonic Therapy; Wound healing; Brazil
• ISSN: 1477-3155; 1477-3155
• DOI: 10.1186/1477-3155-10-11

Nanogold has been investigated in a wide variety of biomedical applications because of the anti-inflammatory properties. The purpose of this study was to evaluate the effects of TPU (Therapeutic Pulsed Ultrasound) with gold nanoparticles (GNP) on oxidative stress parameters and the expression of pro-inflammatory molecules after traumatic muscle injury. Animals were divided in nine groups: sham (uninjured muscle); muscle injury without treatment; muscle injury + DMSO; muscle injury + GNP; muscle injury + DMSO + GNP; muscle injury + TPU; muscle injury + TPU + DMSO; muscle injury + TPU + GNP; muscle injury + TPU + DMSO + GNP. The ROS production was determined by concentration of superoxide anion, modulation of antioxidant defenses was determined by the activity of superoxide dismutase, catalase and glutathione peroxidase enzymes, oxidative damage determined by formation of thiobarbituric acid-reactive substance and protein carbonyls. The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured as inflammatory parameters. Compared to muscle injury without treatment group, the muscle injury + TPU + DMSO + GNP gel group promoted a significant decrease in superoxide anion production and lipid peroxidation levels (p < 0.050). It also showed a significant decrease in TNF-α and IL-1β levels (p < 0.050) when compared to muscle injury without treatment group. Our results suggest that TPU + DMSO + GNP gel presents beneficial effects on the muscular healing process, inducing a reduction in the production of ROS and also the expression of pro-inflammatory molecules.