High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota

• Published in: Gut Vol. 57; no. 11; pp. 1605 - 1615
• Main Authors: Zoetendal, E.G, Rajilic-Stojanovic, M, Vos, W.M. de
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and British Society of Gastroenterology 01.11.2008; BMJ; BMJ Publishing Group LTD
• Subjects: 16s ribosomal-rna; Bacteria; Bacteria - classification; Bacteria - genetics; Bacterial Physiological Phenomena; Biological and medical sciences; Ecosystems; Gastroenterology. Liver. Pancreas. Abdomen; Gastrointestinal Tract - microbiology; Genes; Genes, rRNA - genetics; Genome, Bacterial - genetics; gradient gel-electrophoresis; group-specific primers; human feces; human gut; human intestinal bacteria; Humans; irritable-bowel-syndrome; Medical sciences; Phylogenetics; Phylogeny; rdna sequence-analysis; real-time pcr; RNA, Ribosomal, 16S - analysis; RNA, Ribosomal, 16S - genetics; sp-nov; Digestive tract; Gastroenterology
• ISSN: 0017-5749; 1468-3288
• DOI: 10.1136/gut.2007.133603

The human gastrointestinal (GI) tract microbiota plays a pivotal role in our health. For more than a decade a major input for describing the diversity of the GI tract microbiota has been derived from the application of small subunit ribosomal RNA (SSU rRNA)-based technologies. These not only provided a phylogenetic framework of the GI tract microbiota, the majority of which has not yet been cultured, but also advanced insights into the impact of host and environmental factors on the microbiota community structure and dynamics. In addition, it emerged that GI tract microbial communities are host and GI tract location-specific. This complicates establishing relevant links between the host’s health and the presence or abundance of specific microbial populations and argues for the implementation of novel high-throughput technologies in studying the diversity and functionality of the GI tract microbiota. Here, we focus on the recent developments and applications of phylogenetic microarrays based on SSU rRNA sequences and metagenomics approaches exploiting rapid sequencing technologies in unravelling the secrets of our GI tract microbiota.