Chromatin Interaction Analysis with Paired-End Tag (ChIA-PET) sequencing technology and application

• Published in: BMC genomics Vol. 15 Suppl 12; no. S12; p. S11
• Main Authors: Li, Guoliang, Cai, Liuyang, Chang, Huidan, Hong, Ping, Zhou, Qiangwei, Kulakova, Ekaterina V, Kolchanov, Nikolay A, Ruan, Yijun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.12.2014; BioMed Central
• Subjects: Analysis; Binding sites; Bioinformatics; Biology; Chromatin; Chromatin - chemistry; Chromatin - metabolism; Chromosomes; Data analysis; Deoxyribonucleic acid; DNA; DNA binding proteins; Experiments; Gene expression; Genomes; Genomics; Laboratories; Methods; Promoter Regions, Genetic; Proteins; RNA polymerase; Sequence Analysis, DNA - methods; Technology; Transcription Factors - metabolism; Transcription, Genetic
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/1471-2164-15-S12-S11

Long-range chromatin interactions play an important role in transcription regulation. Chromatin Interaction Analysis with Paired-End-Tag sequencing (ChIA-PET) is an emerging technology that has unique advantages in chromatin interaction analysis, and thus provides insight into the study of transcription regulation. This article introduces the experimental protocol and data analysis process of ChIA-PET, as well as discusses some applications using this technology. It also unveils the direction of future studies based on this technology. Overall we show that ChIA-PET is the cornerstone to explore the three-dimensional (3D) chromatin structure, and certainly will lead the forthcoming wave of 3D genomics studies.