Quantitative proteomics analysis of early recurrence/metastasis of huge hepatocellular carcinoma following radical resection

• Published in: Proteome science Vol. 12; no. 1; p. 22
• Main Authors: Huang, Xinhui, Zeng, Yongyi, Xing, Xiaohua, Zeng, Jinhua, Gao, Yunzhen, Cai, Zhixiong, Xu, Bo, Liu, Xiaolong, Huang, Aimin, Liu, Jingfeng
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01.05.2014; BioMed Central
• Subjects: Analysis; Behavior; Biomarkers; Care and treatment; Chromatography; Complications and side effects; Development and progression; Experiments; Gene expression; Hepatoma; Hospitals; Liver cancer; Medical prognosis; Medical research; Metastasis; Ontology; Peptides; Prognosis; Protein expression; Proteins; Proteomics; Risk factors; Science; China; iTRAQ; Potential prognostic biomarker; Huge hepatocellular carcinoma (H-HCC); Quantitative proteomics; Early recurrence/metastasis
• ISSN: 1477-5956; 1477-5956
• DOI: 10.1186/1477-5956-12-22

Hepatic resection is the preferred treatment for huge hepatocellular carcinoma (>10 cm in diameter; H-HCC). However, the patients with H-HCC suffer from poor prognosis due to the early recurrence/metastasis. The underlying mechanism of H-HCC's early recurrence/metastasis is currently not well understood. Here, we describe an Isobaric Tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics approach to analyze the early recurrence/metastasis related proteins of H-HCC after radical resection through multidimensional chromatography coupled with tandem mass spectrometry (2DLC-MS/MS). The different protein expression profiles between the early recurrence/metastasis within 6 months(R/M≤6months) and late recurrence/metastasis within 6-12 months after surgery (R/M6-12months) were confirmed and might reveal different underlying molecular mechanisms. We identified 44 and 49 significantly differentially expressed proteins in the R/M≤6months group and the R/M6-12months group compared to the group who had no recurrence within 2 years post surgery (the NR/M group), respectively. Moreover, among those proteins, S100A12 and AMACR were down regulated in the R/M≤6months group but up-regulated in the R/M6-12months group; and this regulation was further confirmed in mRNA and protein level by Q-PCR, Western-Blot and Immunohistochemistry (IHC). This current study presents the first proteomic profile of the early recurrence/metastasis of H-HCC. The results suggest that S100A12 and AMACR might be potential prognostic markers for predicting the early recurrence/metastasis of H-HCC after hepatectomy.