Identification of H-2d restricted T cell epitope of foot-and-mouth disease virus structural protein VP1

• Published in: Virology journal Vol. 8; no. 1; p. 426
• Main Authors: Liu, Xin-Sheng, Wang, Yong-Lu, Zhang, Yong-Guang, Fang, Yu-Zhen, Pan, Li, Lu, Jian-Liang, Zhou, Peng, Zhang, Zhong-Wang, Jiang, Shou-Tian
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.09.2011; BioMed Central; BMC
• Subjects: Animals; Antibodies, Viral - immunology; Antigens, Viral - chemistry; Antigens, Viral - genetics; Antigens, Viral - immunology; Blotting, Western; Capsid Proteins - chemistry; Capsid Proteins - genetics; Capsid Proteins - immunology; Cattle; Cell Proliferation; Cloning; Computational Biology; Diagnosis; Enzyme-Linked Immunospot Assay; Epitopes, T-Lymphocyte - chemistry; Epitopes, T-Lymphocyte - genetics; Epitopes, T-Lymphocyte - immunology; Female; Foot-and-mouth disease; Foot-and-Mouth Disease - immunology; Foot-and-Mouth Disease - prevention & control; Foot-and-Mouth Disease - virology; Foot-and-Mouth Disease Virus - chemistry; Foot-and-Mouth Disease Virus - genetics; Foot-and-Mouth Disease Virus - immunology; Health aspects; Immunology; Interferon gamma; Lymphocytes; Lymphocytes - cytology; Lymphocytes - drug effects; Lymphocytes - immunology; Mice; Mice, Inbred BALB C; Peptides - administration & dosage; Peptides - chemical synthesis; Peptides - immunology; Physiological aspects; Proteins; Risk factors; Spleen - cytology; Spleen - drug effects; Spleen - immunology; T cells; Vaccination; Vaccines; Viral infections; China
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-8-426

Foot-and-mouth disease (FMD) is a highly contagious and devastating disease affecting livestock that causes significant financial losses. Therefore, safer and more effective vaccines are required against Foot-and-mouth disease virus(FMDV). The purpose of this study is to screen and identify an H-2d restricted T cell epitope from the virus structural protein VP1, which is present with FMD. We therefore provide a method and basis for studying a specific FMDV T cell epitope. A codon-optimized expression method was adopted for effective expression of VP1 protein in colon bacillus. We used foot-and-mouth disease standard positive serum was used for Western blot detection of its immunogenicity. The VP1 protein was used for immunizing BALB/c mice, and spleen lymphocytes were isolated. Then, a common in vitro training stimulus was conducted for potential H-2Dd, H-2Kd and H-2Ld restricted T cell epitope on VP1 proteins that were predicted and synthesized by using a bioinformatics method. The H-2Kd restricted T cell epitope pK1 (AYHKGPFTRL) and the H-2Dd restricted T cell epitope pD7 (GFIMDRFVKI) were identified using lymphocyte proliferation assays and IFN-γ ELISPOT experiments. The results of this study lay foundation for studying the FMDV immune process, vaccine development, among other things. These results also showed that, to identify viral T cell epitopes, the combined application of bioinformatics and molecular biology methods is effective.