The Comparison of Two Single-cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium
The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver...
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| Published in | Current genomics Vol. 21; no. 8; pp. 602 - 609 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
United Arab Emirates
Bentham Science Publishers Ltd
01.12.2020
Benham Science Publishers Bentham Science Publishers |
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| Abstract | The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them. |
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| AbstractList | The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them.The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them. The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them. |
| Author | Lei Chen Mingnan Zhang Caixia Gao |
| Author_xml | – sequence: 1 givenname: Caixia surname: Gao fullname: Gao, Caixia organization: Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China – sequence: 2 givenname: Mingnan surname: Zhang fullname: Zhang, Mingnan organization: Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China – sequence: 3 givenname: Lei surname: Chen fullname: Chen, Lei organization: Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33414681$$D View this record in MEDLINE/PubMed |
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| Keywords | Single-cell sequencing 10x genomics chromium transcriptome sequencing technology comparison DNA BD rhapsody |
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| Title | The Comparison of Two Single-cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium |
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