The Comparison of Two Single-cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium

The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver...

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Bibliographic Details
Published inCurrent genomics Vol. 21; no. 8; pp. 602 - 609
Main Authors Gao, Caixia, Zhang, Mingnan, Chen, Lei
Format Journal Article
LanguageEnglish
Published United Arab Emirates Bentham Science Publishers Ltd 01.12.2020
Benham Science Publishers
Bentham Science Publishers
Subjects
Chromium
Gene expression
Gene sequencing
Genomics
Medical research
Platforms
Transcription
Single-cell sequencing
10x genomics chromium
transcriptome sequencing
technology comparison
DNA
BD rhapsody
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Summary:The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver of progress in biomedical research. During the last three years, several new single-cell sequencing platforms have emerged. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chromium, including their different mechanisms and some scRNA-seq results obtained with them.
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ISSN:1389-2029
1875-5488
DOI:10.2174/1389202921999200625220812