Immuno-modulatory activity of Ganoderma lucidum-derived polysacharide on human monocytoid dendritic cells pulsed with Der p 1 allergen

• Published in: BMC immunology Vol. 12; no. 1; p. 31
• Main Authors: Jan, Rong-Hwa, Lin, Teng-Yi, Hsu, Ya-Chun, Lee, Shiuh-Sheng, Lo, Shih-Yen, Chang, Mingi, Chen, Li-Kuang, Lin, Yu-Li
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25.05.2011; BioMed Central; BMC
• Subjects: Allergens; Animals; Antigen Presentation - drug effects; Antigens, Dermatophagoides - immunology; Antigens, Dermatophagoides - metabolism; Antigens, Differentiation - metabolism; Arthropod Proteins; asthma; Asthma - immunology; Asthma in children; Cell Differentiation - drug effects; Cells, Cultured; Child; Cysteine Endopeptidases; Cytokines - metabolism; Dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Dendritic Cells - metabolism; Dendritic Cells - pathology; Humans; Immunomodulation; Lymphocyte Activation - drug effects; Monocytes - pathology; Phagocytosis - drug effects; Physiological aspects; Polysaccharides; Polysaccharides - pharmacology; PS-G; Pyroglyphidae; Reishi - immunology; T cells; Th1 Cells - immunology; Th1 Cells - metabolism; Th1 Cells - pathology; Th1-Th2 Balance - drug effects; Th1/Th2 cells; Taiwan
• ISSN: 1471-2172; 1471-2172
• DOI: 10.1186/1471-2172-12-31

Ganoderma lucidum-derived polysaccharide (PS-G) can rapidly and effectively promote the activation and maturation of immature dendritic cells (DCs), suggesting that PS-G possesses the capacity to regulate immune responses. This study aimed to clarify the immunologic effect of PS-G on monocyte-derived dendritic cells (MD-DCs) from asthmatic children allergic to house dust mites. The MD-DCs were stimulated for 24 h with the related allergen, Der p 1, in the presence or absence of PS-G. Cell surface markers and phagocytic capacity were assessed by FACS analysis, and key polarizing cytokines (IL-12 p40, IL-12 p70, IL-6, IL-23, and IL-10) were quantified. The subsequent regulatory effect of pulsed MD-DCs on naïve T cells was evaluated by determining the T-cell cytokine profile. PS-G induced the maturation of MD-DCs and decreased phagocytic capacity, even if pulsed with Der p 1. After incubation with PS-G and Der p 1, MD-DCs produced higher amounts of IL-12 p70, IL-12 p40, IL-6, IL-23, and IL10 than Der p 1-pulsed DCs. Furthermore, type 1 helper T (Th1) cell cytokine (INF-γ) production was highly increased when naïve autologous T cells were co-cultured with Der p 1-pulsed MD-DCs. Naïve T cells stimulated by MD-DCs pulsed with Der p 1 failed to produce proliferation of T-cells, whereas the addition of PS-G to Der p 1 induced a significant proliferation of T-cells similar to that observed with PS-G alone. The presence of PS-G in an allergen pulse promoted allergic MD-DCs to produce IL-12 p70, IL-12 p40, IL-6, IL-23, and IL-10, and exerted an effect on shifting the immune balance towards Th1 in children with allergic asthma.