Hemin-binding DNA structures on the surface of bacteria promote extracellular electron transfer

• Published in: bioRxiv
• Main Authors: Ajunwa, Obinna Markraphael, Salvador Minero, Gabriel Antonio, Sissel Damsbo Jensen, Meyer, Rikke Louise
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 25.12.2024; Cold Spring Harbor Laboratory
• Edition: 1.2
• Subjects: Bacteria; Bacterial infections; Biofilms; Electron transfer; Energy conservation; Environmental DNA; Extracellular matrix; Hemin; Hydrogen peroxide; Microbiology
• ISSN: 2692-8205; 2692-8205
• DOI: 10.1101/2024.12.20.629652

Recent research has shown that bacteria in anoxic layers of Pseudomonas aeruginosa biofilms can respire by transferring electrons to oxygen via extracellular DNA (eDNA) and DNA-binding redox mediators that are unique to this species. In this study, we propose a similar but generic mechanism by which bacteria can transfer electrons via DNA in biofilms, using hemin as a redox-mediator and hemin-binding G-quadruplex (G4) DNA structures in the extracellular matrix. Using Staphylococcus epidermidis as a model organism, voltammetry showed that eDNA and hemin were needed for extracellular electron transfer (EET). Surface-associated G4-DNA formed a complex with hemin, which transferred electrons from the bacteria to an electrode under anoxic conditions. Addition of G4-DNA and hemin to growing biofilms promoted EET which was stable for days. G4-DNA/hemin is also a peroxidase-like DNAzyme, capable of transferring electrons from bacteria to H2O2. G4-DNA were only recently discovered to be abundant in the extracellular matrix of biofilms. We now show that hemin turns these structures into conduits for EET. The study opens the door to new and generic mechanisms for bacterial energy conservation under oxygen-limiting conditions, and for tackling H2O2, a common host defense mechanism against bacterial infections.Competing Interest StatementThe authors have declared no competing interest.Footnotes* A second affiliation for author Obinna Ajunwa is added along with acknowledgement of funding from Center for Electromicrobiology (CEM) as Dr. Ajunwa used the CEM laboraotories during the start-up phase of the project, and also became an integrated member of the center.