Molecular profiling of T-helper immune genes during dengue virus infection

• Published in: Virology journal Vol. 5; no. 1; p. 165
• Main Authors: Chen, Jincheng, Ng, Mary Mah Lee, Chu, Justin Jang Hann
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31.12.2008; BioMed Central; BMC
• Subjects: Antigens, CD - genetics; Antigens, Differentiation, T-Lymphocyte - genetics; Biomarkers; CD4 lymphocytes; Cell Line, Tumor; Chemokine CCL5 - genetics; Dengue - genetics; Dengue - immunology; Dengue viruses; Gene Expression Profiling; Genetic aspects; Hepatocytes - metabolism; Hepatocytes - virology; Humans; Immune response; Interferon Regulatory Factors - genetics; Interleukin-6 - genetics; Lectins, C-Type; Lymphocyte Activation - genetics; Monocytes - metabolism; Monocytes - virology; Physiological aspects; Receptors, CCR3 - genetics; Short Report; T-Lymphocytes, Helper-Inducer - immunology; Toll-Like Receptor 6 - genetics; Up-Regulation; Singapore
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-5-165

In this study, we provide a comprehensive molecular profiling of the involvement of T- helper (Th) genes during dengue virus infection of different cell types. The Th gene profiles of three human cell types (monocytes, T-cells and hepatocytes) were analyzed simultaneously via array-based RT-PCR upon infection with dengue virus. Differential regulation of 41 Th genes was identified and of which 20 of those genes may contribute to immuno-pathogenesis of dengue virus infection by regulating inflammation, thrombocytopenia and vascular permeability. Among the strongly up-regulated genes were the RANTES, CC-CKR3, IRF4, CLEC2C, IL-6 and TLR6, which are potent inducer of inflammation and vascular permeability. Profiling genes obtained from this study may serve as potential biomarkers and the modulation of Th immune responses during dengue virus infection has important implications in disease outcome.