Microsatellite Primer Development for Post Oak, Quercus stellata (Fagaceae)

• Published in: Applications in plant sciences Vol. 2; no. 10; pp. 1400070 - n/a
• Main Authors: Chatwin, Warren B, Carpenter, Kyrie K, Jimenez, Felix R, Elzinga, Dave B, Johnson, Leigh A, Maughan, Peter J
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01.10.2014; John Wiley & Sons, Inc; Wiley
• Subjects: 454 pyrosequencing; alleles; bioinformatics; Cross Timbers; Deoxyribonucleic acid; DNA; DNA primers; ecosystems; Forests; Genetic diversity; Genetic markers; Genetic testing; genetic variation; genomic libraries; Heterozygosity; Kansas; microsatellite repeats; Microsatellites; old-growth forest; old-growth forests; Plant sciences; Polymorphism; Population; Population genetics; Population structure; post oak; Primer Note; PRIMER NOTES; Primers; Quercus stellata; sequence analysis; simple sequence repeat; Terrestrial ecosystems; Texas; Texas; Kansas; United States > US; North America; Oklahoma; Utah; 454 pyrosequencing; Cross Timbers; simple sequence repeat; old-growth forest; Quercus stellata; post oak
• ISSN: 2168-0450; 2168-0450
• DOI: 10.3732/apps.1400070

Premise of the study: The American Cross Timbers forest ecosystem runs from southeastern Kansas to Central Texas and is primarily composed of post oak (Quercus stellata). This old-growth forest currently occupies only about 2% of its ancestral range. To facilitate genetic research on this species, we developed microsatellite primers specific to post oak from reduced genomic libraries. Methods and Results: Two Q. stellata individuals, sampled from the northern and southern range of the post oak forest, were subject to genomic reduction and 454 pyrosequencing. Bioinformatic analysis identified putative microsatellites from which 12 polymorphic primer sets were screened on three populations. The number of alleles observed ranged from five to 20 across all populations, while observed and expected heterozygosity values ranged from 0.05 to 0.833 and 0.236 to 0.893, respectively, within individual populations. Conclusions: We report the development of microsatellite markers, specific to post oak, to aid the study of genetic diversity and population structure of extant forest remnants.