Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer
This study aimed to find novel biomarkers for colorectal cancer. Fluorescent mRNA differential display PCR (DD-PCR) was used to screen the genes differentially expressed in colorectal cancer tissues and their adjacent tissues. The differentially expressed genes were confirmed by real-time PCR and th...
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Published in | Journal of experimental & clinical cancer research Vol. 28; no. 1; p. 11 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
BioMed Central Ltd
22.01.2009
BioMed Central BMC |
Subjects | |
Online Access | Get full text |
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Summary: | This study aimed to find novel biomarkers for colorectal cancer.
Fluorescent mRNA differential display PCR (DD-PCR) was used to screen the genes differentially expressed in colorectal cancer tissues and their adjacent tissues. The differentially expressed genes were confirmed by real-time PCR and then their clinical relevance (such as association with tumor location and lymph gland metastasis) was further investigated.
We identified by DD-PCR a novel RNA helicase, DHX32, which showed higher expression in colorectal cancer tissues than their adjacent tissues, and this result was confirmed by real time RT-PCR. In addition, we found that the level of DHX32 gene expression in colorectal cancer was significantly associated with cancer location, lymph gland metastasis, cancer nodal status, differentiation grade, and Dukes, stage.
DHX32 may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1756-9966 0392-9078 1756-9966 |
DOI: | 10.1186/1756-9966-28-11 |