Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer

• Published in: Journal of experimental & clinical cancer research Vol. 28; no. 1; p. 11
• Main Authors: Huang, Chunling, Liang, Xianming, Huang, Ruxin, Zhang, Zhongying
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 22.01.2009; BioMed Central; BMC
• Subjects: Biological markers; Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Care and treatment; Colorectal cancer; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; DEAD-box RNA Helicases - biosynthesis; DEAD-box RNA Helicases - genetics; Diagnosis; Female; Gene Expression Regulation, Neoplastic; Genetic aspects; Health aspects; Helicases; Humans; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Staging; Polymerase Chain Reaction; Up-Regulation; China
• ISSN: 1756-9966; 0392-9078; 1756-9966
• DOI: 10.1186/1756-9966-28-11

This study aimed to find novel biomarkers for colorectal cancer. Fluorescent mRNA differential display PCR (DD-PCR) was used to screen the genes differentially expressed in colorectal cancer tissues and their adjacent tissues. The differentially expressed genes were confirmed by real-time PCR and then their clinical relevance (such as association with tumor location and lymph gland metastasis) was further investigated. We identified by DD-PCR a novel RNA helicase, DHX32, which showed higher expression in colorectal cancer tissues than their adjacent tissues, and this result was confirmed by real time RT-PCR. In addition, we found that the level of DHX32 gene expression in colorectal cancer was significantly associated with cancer location, lymph gland metastasis, cancer nodal status, differentiation grade, and Dukes, stage. DHX32 may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation.