Splice variants of zinc finger protein 695 mRNA associated to ovarian cancer

Studies of alternative mRNA splicing (AS) in health and disease have yet to yield the complete picture of protein diversity and its role in physiology and pathology. Some forms of cancer appear to be associated to certain alternative mRNA splice variants, but their role in the cancer development and...

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Published inJournal of ovarian research Vol. 6; no. 1; p. 61
Main Authors Juárez-Méndez, Sergio, Zentella-Dehesa, Alejandro, Villegas-Ruíz, Vanessa, Pérez-González, Oscar Alberto, Salcedo, Mauricio, López-Romero, Ricardo, Román-Basaure, Edgar, Lazos-Ochoa, Minerva, Montes de Oca-Fuentes, Víctor Edén, Vázquez-Ortiz, Guelaguetza, Moreno, José
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 05.09.2013
BioMed Central
Subjects
Biomedical research
Cancer
Cancer cells
Development and progression
Gene expression
Genetic aspects
Genomes
Health aspects
Hospitals
Kinases
Medicine
Messenger RNA
Mortality
Oncology
Ovarian cancer
Physiological aspects
Principal components analysis
Proteins
Reproductive health
Science
Mexico
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Summary:Studies of alternative mRNA splicing (AS) in health and disease have yet to yield the complete picture of protein diversity and its role in physiology and pathology. Some forms of cancer appear to be associated to certain alternative mRNA splice variants, but their role in the cancer development and outcome is unclear. We examined AS profiles by means of whole genome exon expression microarrays (Affymetrix GeneChip 1.0) in ovarian tumors and ovarian cancer-derived cell lines, compared to healthy ovarian tissue. Alternatively spliced genes expressed predominantly in ovarian tumors and cell lines were confirmed by RT-PCR. Among several significantly overexpressed AS genes in malignant ovarian tumors and ovarian cancer cell lines, the most significant one was that of the zinc finger protein ZNF695, with two previously unknown mRNA splice variants identified in ovarian tumors and cell lines. The identity of ZNF695 AS variants was confirmed by cloning and sequencing of the amplicons obtained from ovarian cancer tissue and cell lines. Alternative ZNF695 mRNA splicing could be a marker of ovarian cancer with possible implications on its pathogenesis.
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ISSN:1757-2215
1757-2215
DOI:10.1186/1757-2215-6-61