Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS

Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. High performance liquid chromatograp...

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Bibliographic Details
Published inChinese medicine Vol. 6; no. 1; p. 9
Main Authors Yao, Hong, Shi, Peiying, Shao, Qing, Fan, Xiaohui
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 24.02.2011
BioMed Central
BMC
Subjects
Analytical chemistry
Chromatography
Health aspects
High performance liquid chromatography
Mass spectrometry
Saponins
Spectrum analysis
China
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Summary:Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. High performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to identify and quantify the chromatographic fingerprints of the XST injection. Characteristic common peaks were identified using HPLC with photo diode array detection/electrospray ionization tandem mass spectrometry (HPLC-PDA/ESI-MSn). Representative fingerprints from ten batches of samples showed 27 'common saponins' all of which were identified and quantified using ten reference saponins. Chemical fingerprinting and quantitative analysis identified most of the common saponins for the quality control of P. notoginseng products such as the XST injection.
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ISSN:1749-8546
1749-8546
DOI:10.1186/1749-8546-6-9