Analysis of cell-based RNAi screens

RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw da...

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Bibliographic Details
Published inGenome biology Vol. 7; no. 7; p. R66
Main Authors Boutros, Michael, Brás, Lígia P, Huber, Wolfgang
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 25.07.2006
BioMed Central
Subjects
Quality Control
Reproducibility of Results
RNA Interference
Software
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Summary:RNA interference (RNAi) screening is a powerful technology for functional characterization of biological pathways. Interpretation of RNAi screens requires computational and statistical analysis techniques. We describe a method that integrates all steps to generate a scored phenotype list from raw data. It is implemented in an open-source Bioconductor/R package, cellHTS (http://www.dkfz.de/signaling/cellHTS). The method is useful for the analysis and documentation of individual RNAi screens. Moreover, it is a prerequisite for the integration of multiple experiments.
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ISSN:1474-760X
1465-6906
1474-760X
1465-6914
DOI:10.1186/gb-2006-7-7-r66