Identification of the skeletal progenitor cells forming osteophytes in osteoarthritis

• Published in: Annals of the rheumatic diseases Vol. 79; no. 12; pp. 1625 - 1634
• Main Authors: Roelofs, Anke J, Kania, Karolina, Rafipay, Alexandra J, Sambale, Meike, Kuwahara, Stephanie T, Collins, Fraser L, Smeeton, Joanna, Serowoky, Maxwell A, Rowley, Lynn, Wang, Hui, Gronewold, René, Kapeni, Chrysa, Méndez-Ferrer, Simón, Little, Christopher B, Bateman, John F, Pap, Thomas, Mariani, Francesca V, Sherwood, Joanna, Crump, J Gage, De Bari, Cosimo
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd and European League Against Rheumatism 01.12.2020; BMJ Publishing Group LTD
• Subjects: Age; Animal models; Animals; Arthritis; Cartilage; Cartilage (articular); Cartilage diseases; Cell Lineage; Chondrocytes; Embryos; experimental; Experiments; fibroblasts; Growth differentiation factor 5; Hybridization; Immunofluorescence; Knee; Labeling; Meniscus; Mesenchyme; Mice; Osteoarthritis; Osteoarthritis - pathology; Osteophyte - pathology; Osteophytes; Osteoprogenitor cells; Periosteum; Periosteum - pathology; Progenitor cells; Ribonucleic acid; RNA; Sox9 protein; Statistical analysis; Stem cell transplantation; Stem Cells - pathology; Surgery; Synovial Membrane - pathology; Synovium; Tamoxifen; fibroblasts; experimental; chondrocytes; arthritis; osteoarthritis
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/annrheumdis-2020-218350

ObjectivesOsteophytes are highly prevalent in osteoarthritis (OA) and are associated with pain and functional disability. These pathological outgrowths of cartilage and bone typically form at the junction of articular cartilage, periosteum and synovium. The aim of this study was to identify the cells forming osteophytes in OA.MethodsFluorescent genetic cell-labelling and tracing mouse models were induced with tamoxifen to switch on reporter expression, as appropriate, followed by surgery to induce destabilisation of the medial meniscus. Contributions of fluorescently labelled cells to osteophytes after 2 or 8 weeks, and their molecular identity, were analysed by histology, immunofluorescence staining and RNA in situ hybridisation. Pdgfrα-H2BGFP mice and Pdgfrα-CreER mice crossed with multicolour Confetti reporter mice were used for identification and clonal tracing of mesenchymal progenitors. Mice carrying Col2-CreER, Nes-CreER, LepR-Cre, Grem1-CreER, Gdf5-Cre, Sox9-CreER or Prg4-CreER were crossed with tdTomato reporter mice to lineage-trace chondrocytes and stem/progenitor cell subpopulations.ResultsArticular chondrocytes, or skeletal stem cells identified by Nes, LepR or Grem1 expression, did not give rise to osteophytes. Instead, osteophytes derived from Pdgfrα-expressing stem/progenitor cells in periosteum and synovium that are descendants from the Gdf5-expressing embryonic joint interzone. Further, we show that Sox9-expressing progenitors in periosteum supplied hybrid skeletal cells to the early osteophyte, while Prg4-expressing progenitors from synovial lining contributed to cartilage capping the osteophyte, but not to bone.ConclusionOur findings reveal distinct periosteal and synovial skeletal progenitors that cooperate to form osteophytes in OA. These cell populations could be targeted in disease modification for treatment of OA.