A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 108; no. 1; pp. 119 - 122
• Main Authors: Moreira, Luciana Peniche, Watanabe, Aripuanã Sakurada Aranha, Carraro, Emerson, da Silva, Ellen Ricci Monteiro, Guatura, Sandra Baltazar, Granato, Celso, Bellei, Nancy Junqueira
• Format: Journal Article
• Language: English
• Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01.02.2013; Instituto Oswaldo Cruz, Ministério da Saúde; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Adolescent; Adult; Aged; Brazil - epidemiology; Child; Child, Preschool; comparison methods; Fluorescent Antibody Technique, Direct; Hematologic Neoplasms - surgery; Hematopoietic Stem Cell Transplantation - adverse effects; Human respiratory syncytial virus; human stem cell transplant patients; Humans; Infant; Infant, Newborn; Middle Aged; Nasopharynx - virology; PARASITOLOGY; Respiratory syncytial virus; Respiratory Syncytial Virus Infections - diagnosis; Respiratory Syncytial Virus Infections - epidemiology; Respiratory Syncytial Virus, Human - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - analysis; Short Communications; TROPICAL MEDICINE; Young Adult; Brazil; respiratory syncytial virus; comparison methods; human stem cell transplant patients
• ISSN: 1678-8060; 0074-0276; 1678-8060; 0074-0276
• DOI: 10.1590/S0074-02762013000100021

Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy® and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients.