Effects of Gram-positive bacterial pathogens in ewes: peptidoglycan as a potential mediator of interruption of early pregnancy

• Published in: Reproduction (Cambridge, England) Vol. 125; no. 2; pp. 295 - 299
• Main Authors: Stewart, AB, Inskeep, EK, Townsend, EC, Dailey, RA
• Format: Journal Article
• Language: English
• Published: Colchester Society for Reproduction and Fertility 01.02.2003; Portland
• Subjects: Abortion, Septic - blood; Abortion, Septic - veterinary; animal pathogenic bacteria; Animals; Bacterial diseases; Biological and medical sciences; Dinoprost - analogs & derivatives; Dinoprost - blood; Estradiol - blood; Experimental bacterial diseases and models; Female; Fever - blood; Fever - microbiology; Fever - veterinary; Gram-positive bacteria; Hydrocortisone - blood; Infectious diseases; Luteinizing Hormone - blood; Medical sciences; Peptidoglycan - adverse effects; Pregnancy; Progesterone - blood; Sheep; Sheep Diseases - blood; Streptococcal Infections - blood; Streptococcal Infections - veterinary; Streptococcus pyogenes; Animal microorganism relation; Embryo; Cell wall; Endocrine secretion; Gram positive bacteria; Luteinizing hormone; Female; Ungulata; Inflammation; Gonadotropin; Survival; Ovarian hormone; Infection; Pregnancy; Peptidoglycan; Vertebrata; Experimental disease; Mammalia; Adenohypophyseal hormone; Animal; Pathogen strain; Bacteriosis; Sheep; Artiodactyla; Sex steroid hormone
• ISSN: 1470-1626; 1741-7899
• DOI: 10.1530/rep.0.1250295

Bacterial cell walls contain peptidoglycan (PTG), which, among other actions, induces fever. The present experiment evaluated the effects of PTG treatment on early pregnancy and blood plasma concentrations of reproductive hormones. Ewes were injected i.v. with saline or 15, 30 or 60 microg kg(-1) sonicated PTG (Streptococcus pyogenes) on day 5 after mating. Each dose of PTG induced fever. Pregnancy rate at day 25 was not related to incidence of fever but tended to differ among treatments (control, 100%; low, 100%; medium, 67%; high, 60%; P < 0.08). Combined pregnancy rate in ewes from control and low dose groups (100%) was greater than that in ewes from medium and high dose groups (64%, P < 0.01). Ewes with high 13,14-dihydro-15-keto-prostaglandin F(2alpha) (PGFM) concentrations had lower pregnancy rates (6 of 10) than those with low concentrations of PGFM (11 of 11; P < 0.05). Mean cortisol concentrations were higher in treated (2.8 +/- 0.28 microg dl(-1)) than in control (1.1 +/- 0.03 microg dl(-1)) ewes (P < 0.01); the pattern of secretion was biphasic and increased in all treated ewes (P < 0.01). Neither means nor profiles of oestradiol differed with treatment. Mean concentrations and the pattern of concentrations of progesterone were reduced in all treated ewes, as indicated by the time by treatment and linear interaction with treatment (1.2 +/- 0.1 versus 1.6 +/- 0.1 ng ml(-1), P < 0.01). Patterns of LH pulses did not differ from 0 to 4 h or 24 to 28 h after treatment; mean plasma LH concentration was lower in ewes treated with 0, 15 or 30 microg PTG kg(-1) than with 60 microg PTG kg(-1) (P < 0.01). Pregnancy status was not related to plasma concentrations or patterns of LH, oestradiol, progesterone or cortisol. Inflammatory mediators, such as PGF(2alpha), may act directly on the embryo or uterus in ewes treated with PTG.