Characterization of TcSTI-1, a homologue of stress-induced protein-1, in Trypanosoma cruzi
The life cycle of the protozoan Trypanosoma cruzi exposes it to several environmental stresses in its invertebrate and vertebrate hosts. Stress conditions are involved in parasite differentiation, but little is known about the stress response proteins involved. We report here the first characterizat...
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Published in | Memórias do Instituto Oswaldo Cruz Vol. 106; no. 1; pp. 70 - 77 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English Portuguese |
Published |
Brazil
Fundação Oswaldo Cruz, Fiocruz
01.02.2011
Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ) |
Subjects | |
Online Access | Get full text |
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Summary: | The life cycle of the protozoan Trypanosoma cruzi exposes it to
several environmental stresses in its invertebrate and vertebrate
hosts. Stress conditions are involved in parasite differentiation, but
little is known about the stress response proteins involved. We report
here the first characterization of stress-induced protein-1 (STI-1) in
T. cruzi (TcSTI-1). This co-chaperone is produced in response to stress
and mediates the formation of a complex between the stress proteins
HSP70 and HSP90 in other organisms. Despite the similarity of TcSTI-1
to STI-1 proteins in other organisms, its expression profile in
response to various stress conditions, such as heat shock, acidic pH or
nutrient starvation, is quite different. Neither polysomal mRNA nor
protein levels changed in exponentially growing epimastigotes cultured
under any of the stress conditions studied. Increased levels of TcSTI-1
were observed in epimastigotes subjected to nutritional stress in the
late growth phase. Co-immunoprecipitation assays revealed an
association between TcSTI-1 and TcHSP70 in T. cruzi epimastigotes.
Immunolocalization demonstrated that TcSTI-1 was distributed throughout
the cytoplasm and there was some colocalization of TcSTI-1 and TcHSP70
around the nucleus. Thus, TcSTI-1 associates with TcHSP70 and TcSTI-1
expression is induced when the parasites are subjected to stress
conditions during specific growth phase. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1678-8060 0074-0276 1678-8060 0074-0276 |
DOI: | 10.1590/S0074-02762011000100012 |