Statistical design and optimization of single cell oil production from sugarcane bagasse hydrolysate by an oleaginous yeast Rhodotorula sp. IIP-33 using response surface methodology

• Published in: SpringerPlus Vol. 3; no. 1; p. 691
• Main Authors: Bandhu, Sheetal, Dasgupta, Diptarka, Akhter, Jawed, Kanaujia, Pankaj, Suman, Sunil K., Agrawal, Deepti, Kaul, Savita, Adhikari, Dilip K., Ghosh, Debashish
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 25.11.2014; Springer Nature B.V; BioMed Central Ltd
• Subjects: Engineering; Humanities and Social Sciences; multidisciplinary; Rhodotorula; Science; Science (multidisciplinary); Response surface methodology; Lignocellulosic biomass; sp; Yeast lipid; Pentose sugar; Rhodotorula sp
• ISSN: 2193-1801; 2193-1801
• DOI: 10.1186/2193-1801-3-691

Single cell oil production from sugarcane bagasse hydrolysate by oleaginous yeast Rhodotorula sp. IIP-33 was analyzed using a two stage statistical design approach based on Response Surface Methodology. Variables like pentose sugar, (NH 4 ) 2 SO 4 , KH 2 PO 4 , yeast extract, pH and temperature were found to influence lipid production significantly. Under optimized condition in a shake flask, yield of lipid was 2.1199 g with fat coefficient of 7.09 which also resembled ~99% similarity to model predicted lipid production. In this paper we are presenting optimized results for production of non polar lipid which could be later deoxygenated into hydrocarbon. A qualitative analyses of selective lipid samples yielded a varying distribution of free acid ranging from C 6 to C 18 , majoring C 16:0 , C 18:0 and C 18:1 under different fermentation conditions.