Metagenomic Exploration of Plastic Degrading Microbes for Biotechnological Application

• Published in: Current genomics Vol. 21; no. 4; pp. 253 - 270
• Main Authors: Purohit, Jyotika, Chattopadhyay, Anirudha, Teli, Basavaraj
• Format: Journal Article
• Language: English
• Published: Sharjah Bentham Science Publishers Ltd 01.05.2020; Benham Science Publishers; Bentham Science Publishers
• Subjects: Biodegradation; Bioinformatics; Biotechnology; Climate change; Community structure; Environmental risk; Enzymes; Laccase; Marine environment; Metagenomics; Microorganisms; Molecular weight; Plastic debris; Polyamide resins; Polyamides; Polyethylene; Polyethylenes; Polymers; Polypropylene; Polystyrene; Polystyrene resins; Polyurethane; Polyurethane resins; Polyvinyl chloride; Survivability; Waste management
• ISSN: 1389-2029; 1875-5488
• DOI: 10.2174/1389202921999200525155711

Since the last few decades, the promiscuous and uncontrolled use of plastics led to the accumulation of millions of tons of plastic waste in the terrestrial and marine environment. It elevated the risk of environmental pollution and climate change. The concern arises more due to the reckless and unscientific disposal of plastics containing high molecular weight polymers, viz., polystyrene, polyamide, polyvinylchloride, polypropylene, polyurethane, and polyethylene, etc. which are very difficult to degrade. Thus, the focus is now paid to search for efficient, eco-friendly, low-cost waste management technology. Of them, degradation of non-degradable synthetic polymer using diverse microbial agents, viz., bacteria, fungi, and other extremophiles become an emerging option. So far, very few microbial agents and their secreted enzymes have been identified and characterized for plastic degradation, but with low efficiency. It might be due to the predominance of uncultured microbial species, which consequently remain unexplored from the respective plastic degrading milieu. To overcome this problem, metagenomic analysis of microbial population engaged in the plastic biodegradation is advisable to decipher the microbial community structure and to predict their biodegradation potential in situ. Advancements in sequencing technologies and bioinformatics analysis allow the rapid metagenome screening that helps in the identification of total microbial community and also opens up the scope for mining genes or enzymes (hydrolases, laccase, etc.) engaged in polymer degradation. Further, the extraction of the core microbial population and their adaptation, fitness, and survivability can also be deciphered through comparative metagenomic study. It will help to engineer the microbial community and their metabolic activity to speed up the degradation process.