RNA interference and potential applications

RNA interference (RNAi) is the process of using specific sequences of double-stranded RNA (dsRNA) to knock down the expression level of sequence-homologous genes. Such ability of small interfering RNA (siRNA) in mammalian cells will undoubtedly revolutionize the study of functional genomics, the dis...

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Bibliographic Details
Published inCurrent topics in medicinal chemistry Vol. 6; no. 9; p. 901
Main Authors Zhou, Demin, He, Qiuchen S, Wang, Cuiying, Zhang, Jing, Wong-Staal, Flossie
Format Journal Article
LanguageEnglish
Published United Arab Emirates 01.05.2006
Subjects
Animals
Base Sequence
Humans
Plasmids
RNA Interference
RNA, Small Interfering - chemistry
Transcription, Genetic
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Summary:RNA interference (RNAi) is the process of using specific sequences of double-stranded RNA (dsRNA) to knock down the expression level of sequence-homologous genes. Such ability of small interfering RNA (siRNA) in mammalian cells will undoubtedly revolutionize the study of functional genomics, the discovery of drug targets and even the treatment of human diseases. In this review we briefly describe the history of RNAi discovery, the RNAi mechanism and the general guideline for siRNA design as well as various methods for siRNA production and delivery. We also introduce the potential applications of siRNA, inducible siRNA and siRNA library in speeding up basic biomedical research and in acting as potential therapeutic agents for treatment of numerous human diseases.
ISSN:1568-0266
DOI:10.2174/156802606777303630