Development of Tissue Culture Methods for the Rescue and Propagation of Endangered Moringa Spp. Germplasm

Moringa is an Old-World dry tropical plant genus with great food, horticultural, industrial, and pharmaceutical potential. Although many of the thirteen known Moringa species are in danger of extinction, one species, M. oleifera Lam., is now widely cultivated. M. oleifera was therefore utilized to d...

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Bibliographic Details
Published inEconomic botany Vol. 58; no. sp1; pp. S116 - S124
Main Authors Stephenson, Katherine K, Fahey, Jed W
Format Journal Article
LanguageEnglish
Published Heidelberg The New York Botanical Garden Press 01.12.2004
Bronx, NY Springer
New York Botanical Garden Press
Springer Nature B.V
Subjects
Agronomy. Soil science and plant productions
Biological and medical sciences
drumstick tree
Fundamental and applied biological sciences. Psychology
horseradish tree
Immatures
Liquids
Methods
Moringa
nutrition
plant tissue culture
Plant tissues
Plantlets
Plants
Preservatives
Rafts
s
Seed pods
Seedlings
Seeds
Species extinction
Tissue culture techniques
Tropical plants
Plant tissue
Rescue
horseradish tree
Endangered species
Nutrition
Ethnobotany
plant tissue culture
Tissue culture
Multipurpose tree
Moringa
Germplasm
drumstick tree
Dicotyledones
Angiospermae
Moringaceae
Spermatophyta
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Summary:Moringa is an Old-World dry tropical plant genus with great food, horticultural, industrial, and pharmaceutical potential. Although many of the thirteen known Moringa species are in danger of extinction, one species, M. oleifera Lam., is now widely cultivated. M. oleifera was therefore utilized to develop micropropagation techniques that may be applicable to the more endangered members of this genus. Immature seeds were the most responsive tissue source, and greatest success was achieved using membrane rafts and a liquid growth medium. The success rate was 73%, but the multiplication rate averaged only 4.7 shoots per culture. Most vigorous plantlet development through the transplant stage was achieved using a commercial plant preservative formulation of isothiazolones following shoot proliferation. Although there was no evidence of contamination, treatment with this microbiocide prevented early tissue senescence and it increased culture survivability.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0013-0001
1874-9364
DOI:10.1663/0013-0001(2004)58[S116:DOTCMF]2.0.CO;2