MicroRNA-182 modulates chemosensitivity of human non-small cell lung cancer to cisplatin by targeting PDCD4

• Published in: Diagnostic pathology Vol. 9; no. 1; p. 143
• Main Authors: Ning, Fang-ling, Wang, Feng, Li, Mian-li, Yu, Ze-Shun, Hao, Yan-zhang, Chen, Shao-shui
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10.07.2014; BioMed Central
• Subjects: Antineoplastic Agents - pharmacology; Apoptosis Regulatory Proteins - biosynthesis; Biotechnology; Blotting, Western; Cancer therapies; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - metabolism; Cell growth; Cell Line, Tumor; Cisplatin - pharmacology; Drug resistance; Drug Resistance, Neoplasm - genetics; Gene expression; Gene Expression Regulation, Neoplastic - genetics; Humans; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; MicroRNAs; MicroRNAs - genetics; Proteins; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA-Binding Proteins - biosynthesis; Rodents; Signal transduction; Statistical analysis; Studies; Tumorigenesis; China
• ISSN: 1746-1596; 1746-1596
• DOI: 10.1186/1746-1596-9-143

Overexpression of microRNA-182 (miR-182) is found in various human cancers, including non-small cell lung cancer (NSCLC). Our aim is to investigate the association of miR-182 expression with the sensitivity of NSCLC to cisplatin. TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-182 and programmed cell death 4 (PDCD4) protein. miR-182 and (or) PDCD4 depleted cell lines were generated using miR-182 inhibitor and (or) siRNA. The viabilities of treated cells were analyzed using MTT assay. The expression level of miR-182 in A549 cell line was significantly higher than that in NHBE cell line (p < 0.01). Transfection of miR-182 inhibitor induced sensitivity of A549 cells to cisplatin. A549 cells transfected with PDCD4 siRNA became more resistant to cisplatin therapy. We found an increase PDCD4 protein level following the transfection of miR-182 inhibitor using Western blot analysis. In addition, the enhanced growth-inhibitory effect by miR-182 inhibitor was weakened after the addition of PDCD4 siRNA. The results of the present study demonstrated that overexpression of miR-182 may involve in chemoresistance of NSCLC cells to cisplatin by down-regulating PDCD4. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1793467320130186.