Maternal diabetes causes abnormal dynamic changes of endoplasmic reticulum during mouse oocyte maturation and early embryo development

• Published in: Reproductive biology and endocrinology Vol. 11; no. 1; p. 31
• Main Authors: Zhang, Chun-Hui, Qian, Wei-Ping, Qi, Shu-Tao, Ge, Zhao-Jia, Min, Ling-Jiang, Zhu, Xiu-Lang, Huang, Xin, Liu, Jing-Ping, Ouyang, Ying-Chun, Hou, Yi, Schatten, Heide, Sun, Qing-Yuan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.04.2013; BioMed Central
• Subjects: Analysis; Animals; Cell Nucleus - metabolism; Cells, Cultured; Cytoplasm - metabolism; Diabetes; Diabetes Mellitus, Experimental - physiopathology; Embryo, Mammalian - embryology; Embryo, Mammalian - metabolism; Embryonic Development - physiology; Endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Female; Health aspects; Male; Mice; Mice, Inbred ICR; Microscopy, Confocal; Oocytes - cytology; Oocytes - growth & development; Physiological aspects; Pregnancy; Pregnancy Complications - physiopathology; Proteins; Reproductive health; Rodents; Time Factors; Time-Lapse Imaging; Beijing China
• ISSN: 1477-7827; 1477-7827
• DOI: 10.1186/1477-7827-11-31

The adverse effects of maternal diabetes on oocyte maturation and embryo development have been reported. In this study, we used time-lapse live cell imaging confocal microscopy to investigate the dynamic changes of ER and the effects of diabetes on the ER's structural dynamics during oocyte maturation, fertilization and early embryo development. We report that the ER first became remodeled into a dense ring around the developing MI spindle, and then surrounded the spindle during migration to the cortex. ER reorganization during mouse early embryo development was characterized by striking localization around the pronuclei in the equatorial section, in addition to larger areas of fluorescence deeper within the cytoplasm. In contrast, in diabetic mice, the ER displayed a significantly higher percentage of homogeneous distribution patterns throughout the entire ooplasm during oocyte maturation and early embryo development. In addition, a higher frequency of large ER aggregations was detected in GV oocytes and two cell embryos from diabetic mice. These results suggest that the diabetic condition adversely affects the ER distribution pattern during mouse oocyte maturation and early embryo development.