Roles of UndA and MtrC of Shewanella putrefaciens W3-18-1 in iron reduction

• Published in: BMC microbiology Vol. 13; no. 1; p. 267
• Main Authors: Yang, Yunfeng, Chen, Jingrong, Qiu, Dongru, Zhou, Jizhong
• Format: Journal Article
• Language: English
• Published: England BioMed Central 25.11.2013; BioMed Central Ltd
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; BASIC BIOLOGICAL SCIENCES; Binding sites; c-type cytochrome; Crystal structure; Gene Deletion; Genes; Genomes; Genomics; Iron - metabolism; Iron reduction; Microbiology; Multigene Family; Mutagenesis; Oxidation-Reduction; Plasmids; Shewanella oneidensis; Shewanella putrefaciens; Shewanella putrefaciens - genetics; Shewanella putrefaciens - metabolism; Shewanella putrefaciens W3-18-1; Studies
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/1471-2180-13-267

The completion of genome sequencing in a number of Shewanella species, which are most renowned for their metal reduction capacity, offers a basis for comparative studies. Previous work in Shewanella oneidensis MR-1 has indicated that some genes within a cluster (mtrBAC-omcA-mtrFED) were involved in iron reduction. To explore new features of iron reduction pathways, we experimentally analyzed Shewanella putrefaciens W3-18-1 since its gene cluster is considerably different from that of MR-1 in that the gene cluster encodes only four ORFs. Among the gene cluster, two genes (mtrC and undA) were shown to encode c-type cytochromes. The ΔmtrC deletion mutant revealed significant deficiencies in reducing metals of Fe2O3, α-FeO(OH), β-FeO(OH), ferric citrate, Mn(IV) and Co(III), but not organic compounds. In contrast, no deficiency of metal reduction was observed in the ΔundA deletion mutant. Nonetheless, undA deletion resulted in progressively slower iron reduction in the absence of mtrC and fitness loss under the iron-using condition, which was indicative of a functional role of UndA in iron reduction. These results provide physiological and biochemical evidences that UndA and MtrC of Shewanella putrefaciens W3-18-1 are involved in iron reduction.