Induction of physiological acrosome reactions in caput epididymal spermatozoa of mice

Mouse caput spermatozoa are considered immature and thus unable to fertilize oocytes. In this study, we determined whether washing mouse caput spermatozoa increased their ability to acrosome react in response to a physiological stimulus. The results obtained showed that mouse caput spermatozoa incub...

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Bibliographic Details
Published inJournal of reproduction & fertility Vol. 100; no. 1; pp. 219 - 224
Main Authors Biegler, B E, Aarons, D J, George, B C, Poirier, G R
Format Journal Article
LanguageEnglish
Published England Society for Reproduction and Fertility 01.01.1994
Subjects
Acrosome - physiology
Animals
Cells, Cultured
Epididymis - physiology
Exocytosis - physiology
Female
Fertilization - physiology
Male
Mice
Mice, Inbred ICR
Sperm Capacitation - physiology
Zona Pellucida - physiology
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Summary:Mouse caput spermatozoa are considered immature and thus unable to fertilize oocytes. In this study, we determined whether washing mouse caput spermatozoa increased their ability to acrosome react in response to a physiological stimulus. The results obtained showed that mouse caput spermatozoa incubated in Earles' modified medium containing calcium chloride and supplemented with BSA and pyruvate for 1 h at 37°C and then washed acrosome reacted in response to both solubilized zonae and immunoaggregation of a zona binding site. In addition, the material removed from caput spermatozoa by washing blocked induced acrosome reactions of cauda spermatozoa. The data indicate that mouse caput epididymal spermatozoa, if incubated and washed, can undergo physiological acrosome reactions.
ISSN:1470-1626
0022-4251
1741-7899
DOI:10.1530/jrf.0.1000219