Promotion of variant human mammary epithelial cell outgrowth by ionizing radiation: an agent-based model supported by in vitro studies

• Published in: Breast cancer research : BCR Vol. 12; no. 1; p. R11
• Main Authors: Mukhopadhyay, Rituparna, Costes, Sylvain V, Bazarov, Alexey V, Hines, William C, Barcellos-Hoff, Mary Helen, Yaswen, Paul
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01.01.2010; National Library of Medicine - MEDLINE Abstracts; BioMed Central
• Subjects: 60 APPLIED LIFE SCIENCES; Adolescent; Breast - pathology; Breast - radiation effects; Breast cancer; Cancer cells; Cell Proliferation - radiation effects; Cells, Cultured; Development and progression; Dose-Response Relationship, Radiation; Epithelial cells; Epithelial Cells - pathology; Epithelial Cells - radiation effects; Female; Gene Silencing; Genes, p16; Genetic aspects; human mammary epithelial cell; Humans; Middle Aged; Models, Biological; Physiological aspects; population doubling; Radiation; reduction mammoplasty; relative biological effectiveness; Research article; Risk factors; unirradiated control; United States
• ISSN: 1465-542X; 1465-5411; 1465-542X
• DOI: 10.1186/bcr2477

Most human mammary epithelial cells (HMEC) cultured from histologically normal breast tissues enter a senescent state termed stasis after 5 to 20 population doublings. These senescent cells display increased size, contain senescence associated beta-galactosidase activity, and express cyclin-dependent kinase inhibitor, p16INK4A (CDKN2A; p16). However, HMEC grown in a serum-free medium, spontaneously yield, at low frequency, variant (v) HMEC that are capable of long-term growth and are susceptible to genomic instability. We investigated whether ionizing radiation, which increases breast cancer risk in women, affects the rate of vHMEC outgrowth. Pre-stasis HMEC cultures were exposed to 5 to 200 cGy of sparsely (X- or gamma-rays) or densely (1 GeV/amu 56Fe) ionizing radiation. Proliferation (bromodeoxyuridine incorporation), senescence (senescence-associated beta-galactosidase activity), and p16 expression were assayed in subcultured irradiated or unirradiated populations four to six weeks following radiation exposure, when patches of vHMEC became apparent. Long-term growth potential and p16 promoter methylation in subsequent passages were also monitored. Agent-based modeling, incorporating a simple set of rules and underlying assumptions, was used to simulate vHMEC outgrowth and evaluate mechanistic hypotheses. Cultures derived from irradiated cells contained significantly more vHMEC, lacking senescence associated beta-galactosidase or p16 expression, than cultures derived from unirradiated cells. As expected, post-stasis vHMEC cultures derived from both unirradiated and irradiated cells exhibited more extensive methylation of the p16 gene than pre-stasis HMEC cultures. However, the extent of methylation of individual CpG sites in vHMEC samples did not correlate with passage number or treatment. Exposure to sparsely or densely ionizing radiation elicited similar increases in the numbers of vHMEC compared to unirradiated controls. Agent-based modeling indicated that radiation-induced premature senescence of normal HMEC most likely accelerated vHMEC outgrowth through alleviation of spatial constraints. Subsequent experiments using defined co-cultures of vHMEC and senescent cells supported this mechanism. Our studies indicate that ionizing radiation can promote the outgrowth of epigenetically altered cells with pre-malignant potential.