Chondroitin and keratan sulphate epitopes, glycosaminoglycans, and hyaluronan in progressive versus non-progressive osteoarthritis

• Published in: Annals of the rheumatic diseases Vol. 56; no. 2; pp. 119 - 122
• Main Authors: Fawthrop, Fiona, Yaqub, Reehana, Belcher, Carolyn, Bayliss, Michael, Ledingham, Joanna, Doherty, Michael
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and European League Against Rheumatism 01.02.1997; BMJ; BMJ Publishing Group LTD
• Subjects: Aged; Aged, 80 and over; Arthritis; Biological and medical sciences; Biomarkers - analysis; Cartilage; Chondroitin Sulfates - analysis; Chondroitin Sulfates - immunology; chondroitin sulphate; Concise Reports; Disease Progression; Diseases of the osteoarticular system; Epitopes - analysis; Female; Follow-Up Studies; Glycosaminoglycans - analysis; Humans; hyaluronan; Hyaluronic acid; Hyaluronic Acid - analysis; Inflammatory joint diseases; Keratan Sulfate - analysis; Keratan Sulfate - immunology; keratan sulphate; Knee; Knee Joint - chemistry; Male; Medical sciences; Middle Aged; Monoclonal antibodies; Osteoarthritis; Osteoarthritis - pathology; Patients; Sodium; synovial fluid; Synovial Fluid - chemistry; Knee; Human; Antigenic determinant; Keratan sulfate; Diseases of the osteoarticular system; Lower limb; Arthritis; Joint; Assay; Progressive; Synovial fluid; Arthropathy; Glycosaminoglycan; Chondroitin sulfate; Hyaluronic acid; Comparative study
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.56.2.119

OBJECTIVE To determine if a single time point estimation of chondroitin sulphate (CS) or keratan sulphate (KS) epitopes, hyaluronan (HA), or total glycosaminoglycans (GAG) in knee synovial fluid at time of hospital referral can predict subsequent radiographic progression of knee osteoarthritis. METHODS Two groups of hospital referred patients with knee osteoarthritis were compared: (1) a “progressive” group (n = 45), showing further reduction in radiographic joint space of at least one grade (0-3) in at least one compartment; and (2) a “non-progressive” group (n = 25) in whom radiographs showed no change during the mean follow up period of 2.3 years (median 2, range 1 to 5 years). Knee synovial fluid obtained at the first visit was examined by elisa for: CS epitopes, using monoclonal antibodies 3B3 and 7D4; KS epitope, using monoclonal antibody 5D4; and HA, using biotinylated HA binding region of cartilage proteoglycan. Total sulphated GAG were measured by dye binding with 1:9 dimethylmethylene blue. RESULTS In patients with bilateral synovial fluid data right and left knee values were closely correlated for all variables. There were no significant differences between CS and KS epitopes, HA, total sulphated GAG, or ratios of individual CS or KS epitopes to total GAG, between progressive and non-progressive groups. CONCLUSIONS Single time point estimation of CS, KS, HA, or total GAG in synovial fluid does not distinguish radiographically progressive and non-progressive knee osteoarthritis patients followed for two years.