Leishmania infection in humans, dogs and sandflies in a visceral leishmaniasis endemic area in Maranhão, Brazil

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 106; no. 2; pp. 207 - 211
• Main Authors: Felipe, Ilana Mirian Almeida, de Aquino, Dorlene Maria Cardoso, Kuppinger, Oliver, Santos, Max Diego Cruz, Rangel, Maurício Eduardo Salgado, Barbosa, David Soeiro, Barral, Aldina, Werneck, Guilherme Loureiro, de Jesus Mendes Caldas, Arlene
• Format: Journal Article
• Language: English
• Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01.03.2011; Instituto Oswaldo Cruz, Ministério da Saúde; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Animals; Brazil; Brazil - epidemiology; Canis familiaris; Dog Diseases - diagnosis; Dog Diseases - epidemiology; Dogs; Endemic Diseases; Enzyme-Linked Immunosorbent Assay; Female; Fluorescent Antibody Technique, Indirect; Humans; Insect Vectors - parasitology; Leishmania - isolation & purification; Leishmania chagasi; Leishmaniasis, Visceral - diagnosis; Leishmaniasis, Visceral - epidemiology; Leishmaniasis, Visceral - veterinary; Lutzomyia longipalpis; Male; Maranhão; PARASITOLOGY; Polymerase Chain Reaction; Psychodidae - parasitology; Seasons; serum prevalence; TROPICAL MEDICINE; visceral leishmaniasis; visceral leishmaniasis - Maranhão; Brazil; Brazil, Maranhao; Maranhão; Brazil; visceral leishmaniasis; serum prevalence; Lutzomyia longipalpis
• ISSN: 1678-8060; 0074-0276; 1678-8060; 0074-0276
• DOI: 10.1590/S0074-02762011000200015

Leishmania infection in humans, dogs and sandflies was examined in the endemic visceral leishmaniasis (VL) municipality of Raposa, state of Maranhão, Brazil. In this study, we examined Leishmania chagasi infection in the blood serum of both humans and Canis familiaris and the natural Leishmania sp. infection rate in the sandfly vector, Lutzomyia longipalpis . Enzyme-linked immunosorbent assay, indirect immunofluorescence reaction and polymerase chain reaction were performed to detect Leishmania infections in humans, dogs and sandflies, respectively. Overall, 186 out of 986 studied human beings were infected with L. chagasi parasites, representing an infection prevalence of 18.9%. An even higher infection rate was detected in dogs, where 66 (47.8%) out of 138 were infected. Among all Lu. longipalpis captured (n = 1,881), only 26.7% were females. The Leishmania infection frequency for the vector Lu. longipalpis was 1.56%. Remarkably, all infected sandflies were found in the peridomiciliary area. Furthermore, a high incidence of asymptomatic forms of VL in the human and canine populations was observed. The results of this study suggest autochthonous transmission of L. chagasi in this endemic area for visceral leishmaniasis because infection by Leishmania sp. was identified in all important elements of the transmission chain