Little evidence for association of the glaucoma gene MYOC with open-angle glaucoma
Background/aimTo determine if overexpression of the glaucoma gene MYOC is involved in the development of open-angle glaucoma (OAG) and if its promoter variants are associated with glaucoma in the Korean population.MethodsHuman trabecular meshwork cells were cultured in the presence of ophthalmic ste...
Saved in:
Published in | British journal of ophthalmology Vol. 94; no. 5; pp. 639 - 642 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
BMA House, Tavistock Square, London, WC1H 9JR
BMJ Publishing Group Ltd
01.05.2010
BMJ Publishing Group BMJ Publishing Group LTD BMJ Group |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Background/aimTo determine if overexpression of the glaucoma gene MYOC is involved in the development of open-angle glaucoma (OAG) and if its promoter variants are associated with glaucoma in the Korean population.MethodsHuman trabecular meshwork cells were cultured in the presence of ophthalmic steroids such as fluorometholone, fluorometholone acetate, dexamethasone, prednisolone acetate and rimexolone. The cells were cultured at a hydrostatic pressure of 32 mm Hg above atmospheric pressure and induction of MYOC was evaluated by northern blot analysis. Genomic DNA was extracted from blood samples obtained from 74 normal controls and 168 unrelated Korean patients with OAG, including primary OAG, normal tension glaucoma and steroid-induced glaucoma. A 461 base pair (bp) DNA fragment of the MYOC promoter region was amplified using PCR and its genotype was analysed by directly sequencing the product.ResultsThe potencies of steroid eye drops in MYOC induction in vitro was the same regardless of their potential for elevating intraocular pressure in vivo. Hydrostatic pressure had no effect on MYOC induction. A dinucleotide repeat polymorphism and three single nucleotide polymorphisms were identified, but no obvious differences in the genotype distribution and allele frequency of the variants between the control group and any type of OAG were observed.ConclusionOur data suggest that MYOC overexpression is not a cause or an effect of intraocular pressure elevation and that MYOC itself is not associated with OAG. |
---|---|
Bibliography: | istex:CFD0E90B82FA18F076982DBEF86E913ECEC938B3 href:bjophthalmol-94-639.pdf ark:/67375/NVC-Q83K0MNB-3 local:bjophthalmol;94/5/639 ArticleID:bjophthalmol158261 |
ISSN: | 0007-1161 1468-2079 |
DOI: | 10.1136/bjo.2009.158261 |