High-throughput mRNA-seq atlas of human placenta shows vast transcriptome remodeling from first to third trimester

• Published in: Biology of reproduction Vol. 110; no. 5; pp. 936 - 949
• Main Authors: Gonzalez, Tania L, Wertheimer, Sahar, Flowers, Amy E, Wang, Yizhou, Santiskulvong, Chintda, Clark, Ekaterina L, Jefferies, Caroline A, Lawrenson, Kate, Chan, Jessica L, Joshi, Nikhil V, Zhu, Yazhen, Tseng, Hsian-Rong, Karumanchi, S. Ananth, Williams, John, Pisarska, Margareta D
• Format: Journal Article
• Language: English
• Published: United States Society for the Study of Reproduction 09.05.2024; Oxford University Press
• Subjects: Adult; Biomarkers; chorionic villi; Environmental factors; Female; Fetuses; Gene expression; gene expression regulation; Genetic analysis; Genetic variability; Gestation; gestational differences; High-Throughput Nucleotide Sequencing; human transcriptome; Humans; mRNA; Next-generation sequencing; Placenta; Placenta - metabolism; Polyadenylation; Pregnancy; Pregnancy Trimester, First - genetics; Pregnancy Trimester, Third - genetics; RESEARCH ARTICLE; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcriptome; Transcriptomes; placenta; gestational differences; mRNA; human transcriptome; chorionic villi; pregnancy
• ISSN: 0006-3363; 1529-7268; 1529-7268
• DOI: 10.1093/biolre/ioae007

The placenta, composed of chorionic villi, changes dramatically across gestation. Understanding differences in ongoing pregnancies are essential to identify the role of chorionic villi at specific times in gestation and develop biomarkers and prognostic indicators of maternal–fetal health. The normative mRNA profile is established using next-generation sequencing of 124 first trimester and 43 third trimester human placentas from ongoing healthy pregnancies. Stably expressed genes (SEGs) not different between trimesters and with low variability are identified. Differential expression analysis of first versus third trimester adjusted for fetal sex is performed, followed by a subanalysis with 23 matched pregnancies to control for subject variability using the same genetic and environmental background. Placenta expresses 14,979 polyadenylated genes above sequencing noise (transcripts per million > 0.66), with 10.7% SEGs across gestation. Differentially expressed genes (DEGs) account for 86.7% of genes in the full cohort [false discovery rate (FDR) < 0.05]. Fold changes highly correlate between the full cohort and subanalysis (Pearson = 0.98). At stricter thresholds (FDR < 0.001, fold change > 1.5), there remains 50.1% DEGs (3353 upregulated in first and 4155 upregulated in third trimester). This is the largest mRNA atlas of healthy human placenta across gestation, controlling for genetic and environmental factors, demonstrating substantial changes from first to third trimester in chorionic villi. Specific differences and SEGs may be used to understand the specific role of the chorionic villi throughout gestation and develop first trimester biomarkers of placental health that transpire across gestation, which can be used for future development of biomarkers for maternal–fetal health. Summary Sentence Comparison of first versus third trimester human placenta mRNA finds a subset of SEGs as well as significant changes in 86.7% of transcripts, highlighting the dynamic nature of placental function across gestation. Graphical Abstract