Observations on Cryptosporidium Life Cycle Stages During Excystation

• Published in: The Journal of parasitology Vol. 94; no. 1; pp. 298 - 300
• Main Authors: Karanis, Panagiotis, Kimura, Akio, Nagasawa, Hideyuki, Igarashi, Ikuo, Suzuki, Naoyoshi
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01.02.2008; Allen Press Inc
• Subjects: Adult; Animals; Axenic culture; Biological and medical sciences; Bleaches; Cryptosporidium; Cryptosporidium parvum; Cryptosporidium parvum - growth & development; Cryptosporidium parvum - physiology; Cryptosporidium parvum - ultrastructure; Developmental biology; Developmental stages; Female; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Genotype & phenotype; Genotypes; Humans; Invertebrates; Life cycle; Life Cycle Stages; Life cycles; Merozoites; Mice; Mice, SCID; Microscopy; Microscopy, Confocal; Oocysts; Parasites; Parasitology; Polymorphism; Propagation; Protozoa; Research Notes; Sporozoites; Japan; Excystment; Protozoa; Cryptosporidium; Life cycle; Apicomplexa; Parasite; Developmental stage
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-1185.1

Cryptosporidium parvum (HNJ-1 strain, genotype 2) merozoites were released from oocysts directly during an incubation and excystation procedure without bleach treatment. They were polymorphic, mostly spindle-shaped; others were bean shaped, actively motile, and underwent division. Merozoites survived for short time–period in an in vitro culture system, but could not be established in a subsequent cultivation effort in RPMI medium.