Expression of CD45RO on circulating CD19+ B-cells in Crohn's disease

• Published in: Gut Vol. 34; no. 12; pp. 1698 - 1704
• Main Authors: Yacyshyn, B R, Pilarski, L M
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and British Society of Gastroenterology 01.12.1993; BMJ; BMJ Publishing Group LTD
• Subjects: Antigens, CD - analysis; Antigens, CD19; Antigens, Differentiation, B-Lymphocyte - analysis; B-Lymphocytes - immunology; Biological and medical sciences; Celiac Disease - immunology; Colitis, Ulcerative - immunology; Crohn Disease - immunology; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Leukocyte Common Antigens - analysis; Leukocyte Count; Medical sciences; Other diseases. Semiology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; T-Lymphocytes - immunology; Antigen; Human; Crohn disease; Phenotype; T-Lymphocyte; Digestive diseases; Intestinal disease; B-Lymphocyte; Cell differentiation; Inflammatory disease
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.34.12.1698

Crohn's disease is an immunoregulatory disorder of the intestine that can be associated with systemic manifestations. This study analysed B-cell differentiation antigens to identify B-cell subpopulations unique to patients with Crohn's disease. CD45 isoform expression was used as an indicator of B-cell differentiation stage. This work shows that B-cells in blood and gut of patients with Crohn's disease are at an advanced stage of differentiation based on their unusual presentation of transitional (RA+ RO+) and late stage (RO+)CD45 isoforms on lamina propria lymphocytes, whereas normal intestinal lamina propria lymphocytes B-cells express primarily CD45RA. Crohn's disease patients had heightened expression of the CD45RO isoform on CD19+ lamina propria lymphocytes, and was found in a statistically significant proportion of Crohn's peripheral blood mononuclear cells (PBMC) where CD19+ PBMC had an expression pattern affecting an unexpectedly high proportion of these differentiated or late stage CD45RO+ B-cells. The expression of CD45RO varied greatly among CD19+ PBMC from patients with Crohn's disease, so multiple regression analysis was performed between these CD45 isoforms and several clinical parameters. After grouping high and low CD45RO expression on CD19+ B-cells, a significant statistical difference was found between high Crohn's disease activity index (CDAI) and low CDAI Crohn's disease patients respectively.