Different DNA changes in primary and recurrent hepatocellular carcinoma

• Published in: Gut Vol. 33; no. 10; pp. 1433 - 1435
• Main Authors: Ding, S F, Jalleh, R P, Wood, C B, Bowles, L, Delhanty, J D, Dooley, J, Habib, N A
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and British Society of Gastroenterology 01.10.1992; BMJ; BMJ Publishing Group LTD
• Subjects: Aged; Autoradiography; Biological and medical sciences; Carcinoma, Hepatocellular - genetics; Chromosome Deletion; DNA Probes; DNA, Neoplasm - genetics; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Liver Neoplasms - genetics; Male; Medical sciences; Neoplasm Recurrence, Local - genetics; Polymorphism, Restriction Fragment Length; Human; Adult; Exploration; Hepatic disease; Molecular biology; Liver cell carcinoma
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.33.10.1433

DNA restriction fragment length polymorphism analysis was carried out on a primary and recurrent hepatocellular carcinoma in a hepatitis B virus negative patient. For the primary tumour, allele losses were found on the short arm of chromosome 17 (probe: p144-D6, 17p13) and the long arm of chromosome 5 with the probe Lambda MS8 (5q35-qter); other probes showed either no allele loss or a non-informative pattern. The recurrent cancer also showed allele loss with p144-D6, but not with Lambda MS8. In addition, the recurrent tumour had allele losses with Lambda MS43 (12q24.3-qter), pYNZ22 (17p13), and DNA rearrangement revealed by the probe Lambda MS32 (1q42-43), a pattern not seen in the primary lesion. These results indicate that the second hepatocellular carcinoma was of independent clonality and probably represents a de novo neoplasm rather than a recurrence.