Insulin restores GH responsiveness during lactation-induced negative energy balance in dairy cattle: effects on expression of IGF-I and GH receptor 1A

• Published in: Journal of endocrinology Vol. 176; no. 2; pp. 205 - 217
• Main Authors: Butler, ST, Marr, AL, Pelton, SH, Radcliff, RP, Lucy, MC, Butler, WR
• Format: Journal Article
• Language: English
• Published: Colchester BioScientifica 01.02.2003; Portland Press
• Subjects: Animals; Biological and medical sciences; Blood Glucose - analysis; Blotting, Western - methods; Cattle; Energy Metabolism - drug effects; Female; Fundamental and applied biological sciences. Psychology; Growth Hormone - blood; Infusions, Intravenous; Insulin - blood; Insulin - pharmacology; Insulin-Like Growth Factor Binding Proteins - analysis; Insulin-Like Growth Factor I - analysis; Insulin-Like Growth Factor I - metabolism; Lactation - physiology; Mother. Fetoplacental unit. Mammary gland. Milk; Pregnancy. Parturition. Lactation; Receptors, Somatotropin - genetics; Receptors, Somatotropin - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Time Factors; Vertebrates: reproduction; Pancreatic hormone; Energy metabolism; Adipose tissue; Lactation; Digestive system; Liver; Dairy cattle; Somatotropin hormone; Ox; Gene expression; Insulin like growth factor 1; Insulin; Vertebrata; Mammalia; Adenohypophyseal hormone; Artiodactyla; Ungulata; Hormonal receptor
• ISSN: 0022-0795; 1479-6805
• DOI: 10.1677/joe.0.1760205

Early lactation in dairy cattle is a period of severe negative energy balance (NEB) characterized by reduced blood glucose and insulin concentrations and elevated blood GH concentrations. The liver is refractory to GH during NEB and this uncoupling of the GH-IGF axis results in diminished plasma concentrations of IGF-I. Our objectives were to examine the effects of insulin administration during the immediate postpartum period on plasma IGF-I and GH concentrations and to examine the hepatic expression of total GH receptors (all GH receptor transcripts), GH receptor 1A (GHR 1A) and IGF-I. In addition, we examined adipose tissue for total GH receptor and IGF-I mRNA levels to establish the effects of chronic hyperinsulinemia on an insulin-responsive peripheral tissue. Holstein cows (n=14) were subjected to either a hyperinsulinemic-euglycemic clamp (insulin; INS) or saline infusion (control; CTL) for 96 h starting on day 10 postpartum. Insulin was infused i.v. (1 micro g/kg body weight per h), blood samples were collected hourly, and euglycemia was maintained by infusion of glucose. Insulin concentrations during the infusions were increased 8-fold in INS compared with CTL cows (2.33+/-0.14 vs 0.27+/-0.14 ng/ml (S.E.M.); P<0.001) while blood glucose concentrations were not different between treatments (45.3+/-2.2 vs 42.5+/-2.2 mg/dl; P>0.1). Plasma IGF-I increased continuously during the insulin infusion, and reached the highest concentrations at the end of the clamp, being almost 4-fold higher in INS compared with CTL cows (117+/-4 vs 30+/-4 ng/ml; P<0.001). Hepatic expression of GHR 1A and IGF-I mRNA was low in CTL cows, but was increased 3.6-fold (P<0.05) and 6.3-fold (P<0.001) respectively in INS cows. By contrast, in adipose tissue the changes in gene expression in response to insulin were reversed with decreases in both total GHR and IGF-I mRNA. The expressions of GHR 1A and IGF-I mRNA in liver tissue were correlated in INS (r=0.86; P<0.05), but not CTL cows (r=0.43; P>0.1). Insulin appears to be a key metabolic signal in coupling the GH-IGF axis, thus orchestrating a marked elevation in circulating IGF-I concentrations.