Genomic presence of recombinant porcine endogenous retrovirus in transmitting miniature swine

The replication of porcine endogenous retrovirus (PERV) in human cell lines suggests a potential infectious risk in xenotransplantation. PERV isolated from human cells following cocultivation with porcine peripheral blood mononuclear cells is a recombinant of PERV-A and PERV-C. We describe two diffe...

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Published inVirology journal Vol. 3; no. 1; p. 91
Main Authors Martin, Stanley I, Wilkinson, Robert, Fishman, Jay A
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 02.11.2006
BioMed Central
BMC
Subjects
Amino Acid Sequence
Animals
Cell Line
Endogenous Retroviruses - genetics
Endogenous Retroviruses - isolation & purification
Endogenous Retroviruses - physiology
Gene Products, env - chemistry
Gene Products, env - genetics
Genome, Viral
Humans
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - virology
Lymphocyte Activation
Molecular Sequence Data
Recombination, Genetic
Short Report
Swine
Swine, Miniature - genetics
Swine, Miniature - virology
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Summary:The replication of porcine endogenous retrovirus (PERV) in human cell lines suggests a potential infectious risk in xenotransplantation. PERV isolated from human cells following cocultivation with porcine peripheral blood mononuclear cells is a recombinant of PERV-A and PERV-C. We describe two different recombinant PERV-AC sequences in the cellular DNA of some transmitting miniature swine. This is the first evidence of PERV-AC recombinant virus in porcine genomic DNA that may have resulted from autoinfection following exogenous viral recombination. Infectious risk in xenotransplantation will be defined by the activity of PERV loci in vivo.
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ISSN:1743-422X
1743-422X
DOI:10.1186/1743-422X-3-91