A diet containing soybean oil heated for three hours increases adipose tissue weight but decreases body weight in C57BL/6 J mice

• Published in: Lipids in health and disease Vol. 12; no. 1; p. 26
• Main Authors: Penumetcha, Meera, Schneider, Mary K, Cheek, Holly A, Karabina, Sonia
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 06.03.2013; BioMed Central
• Subjects: Adipose Tissue - drug effects; Adipose Tissue - metabolism; Adipose tissues; Analysis; Animals; Body Composition - drug effects; Body fat; Body weight; Body Weight - drug effects; Comparative analysis; Cooking; Diet; Diet, Fat-Restricted; Dietary Fats - administration & dosage; Eating - drug effects; Energy Intake - drug effects; Gene Expression - drug effects; Health aspects; Hot Temperature; Human health and pathology; Humans; Ion Channels - genetics; Ion Channels - metabolism; Life Sciences; Lipids; Low density lipoproteins; Male; Mice; Mice, Inbred C57BL; Mitochondrial Proteins - genetics; Mitochondrial Proteins - metabolism; Oils & fats; Physiological aspects; PPAR gamma - genetics; PPAR gamma - metabolism; Proteins; Reducing diets; Short Report; Soy oil; Soybean Oil - chemistry; Soybean Oil - pharmacology; Uncoupling Protein 1; Vegetable oils; Weight Gain - drug effects
• ISSN: 1476-511X; 1476-511X
• DOI: 10.1186/1476-511X-12-26

Our previous work showed that dietary oxidized linoleic acid given, as a single fatty acid, to LDL receptor knockout mice decreased weight gain as compared to control mice. Other studies have also reported that animals fed oils heated for 24 h or greater showed reduced weight gain. These observations, while important, have limited significance since fried foods in the typical human diet do not contain the extreme levels of oxidized lipids used in these studies. The main goal of this study was to investigate the effects of a diet containing soybean oil heated for 3 h on weight gain and fat pad mass in mice. Additionally, because PPARγ and UCP-1 mediate adipocyte differentiation and thermogenesis, respectively, the effect of this diet on these proteins was also examined. Four to six week old male C57BL/6 J mice were randomly divided into three groups and given either a low fat diet with heated soybean oil (HSO) or unheated soybean oil (USO) or pair fed for 16 weeks. Weight and food intake were monitored and fat pads were harvested upon the study's termination. Mice consuming the HSO diet had significantly increased fat pad mass but gained less weight as compared to mice in the USO group despite a similar caloric intake and similar levels of PPARγ and UCP1. This is the first study to show that a diet containing soybean oil heated for a short time increases fat mass despite a decreased weight gain in C57BL/6 J mice. The subsequent metabolic consequences of this increased fat mass merits further investigation.