The level of BLyS (BAFF) correlates with the titre of autoantibodies in human Sjögren’s syndrome

• Published in: Annals of the rheumatic diseases Vol. 62; no. 2; pp. 168 - 171
• Main Authors: Mariette, X, Roux, S, Zhang, J, Bengoufa, D, Lavie, F, Zhou, T, Kimberly, R
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and European League Against Rheumatism 01.02.2003; BMJ; BMJ Publishing Group LTD
• Subjects: Antibodies, Antinuclear - blood; Antigens; autoantibodies; Autoantibodies - blood; Autoimmune diseases; Autoimmune Diseases - immunology; B lymphocyte stimulator; B-Cell Activating Factor; Biological and medical sciences; BLyS; bovine serum albumin; BSA; Disease; ELISA; enzyme linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Extended Report; horseradish peroxidase; HRP; Humans; Immunoglobulins; Immunoglobulins - blood; Lupus; Medical sciences; Membrane Proteins - blood; Patients; PBS; phosphate buffered saline; rheumatoid arthritis; Rheumatoid Factor - blood; Rodents; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Sjogren's Syndrome - immunology; Sjögren’s syndrome; SLE; Statistical analysis; systemic lupus erythematosus; TNF; Tumor Necrosis Factor-alpha; Tumor necrosis factor-TNF; tumour necrosis factor; Autoimmunity; Human; Immunopathology; Biochemical analysis; Antibody; Stomatology; Pathogenesis; Autoantibody; Autoimmune disease; Cellular immunity; B-Lymphocyte; Sjögren syndrome; Immunological method; Eye disease; Systemic disease; Serum; Humoral immunity
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.62.2.168

Background: Increased levels of B lymphocyte stimulator (BLyS) have been detected in serum from patients with systemic lupus erythematosus and rheumatoid arthritis. Objective: To determine the level of BLyS in serum from patients with primary’s Sjögren’s syndrome (SS), another autoimmune disease in which B cell activation is high. Methods: Serum samples from 49 patients with primary SS according to the revised European criteria were assayed for BLyS, quantitative immunoglobulins, and autoantibody levels and compared with samples from 47 healthy control subjects. Results: The median level of BLyS was 5.99 ng/ml (25th-75th centile range 3.20–8.93 ng/ml) in SS v 2.49 ng/ml (25th-75th centile range 1.96–2.96 ng/ml) in healthy controls (p<0.001). More importantly, among patients with SS, the presence of anti-SSA antibodies was associated with significantly higher levels of BLyS (medians 7.90 ng/ml v 3.70 ng/ml; p=0.008) as was the presence of anti-SSB antibodies (medians 7.14 ng/ml v 3.70 ng/ml; p=0.02) and of rheumatoid factor (medians 7.70 ng/ml v 3.80 ng/ml; p=0.016). The level of BLyS in three patients with a monoclonal gammopathy was higher than in the other patients (medians 26.53 ng/ml v 5.92 ng/ml; p=0.13). Higher levels of BLyS were associated with higher levels of gammaglobulins and IgG. There was a strong correlation between BLyS and rheumatoid factor level (r=0.71, p<0.0001), anti-SSA IgG level (r=0.32, p=0.02) and anti-SSA IgM level (r=0.39, p=0.006). Conclusion: In human SS the level of BLyS correlates with the level of autoantibodies. Thus, BLyS may play a part in activating specific autoreactive B cells and modulating the level of production of autoantibodies which are the hallmark of the disease. These findings raise the possibility of a novel therapeutic approach in human SS.