No detection of enteroviral genome in the myocardium of patients with arrhythmogenic right ventricular cardiomyopathy

• Published in: Journal of clinical pathology Vol. 53; no. 5; pp. 382 - 387
• Main Authors: Calabrese, Fiorella, Angelini, Annalisa, Thiene, Gaetano, Basso, Cristina, Nava, Andrea, Valente, Marialuisa
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.05.2000; BMJ; BMJ Publishing Group LTD
• Subjects: Adult; Aged; arrhythmogenic right ventricular cardiomyopathy; Arrhythmogenic Right Ventricular Dysplasia - pathology; Arrhythmogenic Right Ventricular Dysplasia - virology; Biological and medical sciences; Biopsy; Cardiology. Vascular system; Chromosomes; Endocardium - pathology; Endocardium - virology; enterovirus; Enterovirus - genetics; Enterovirus - isolation & purification; Female; Genome, Viral; Heart; Humans; Male; Medical sciences; Middle Aged; molecular biology; myocarditis; Myocarditis. Cardiomyopathies; Polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - analysis; Sensitivity and Specificity; Studies; Viral infections; Human; Immunohistochemistry; Adenoviridae; Arrhythmia; Cardiomyopathy; Right ventricle; Reverse transcription; Cardiovascular disease; Myocardial disease; Virus; Infection; Polymerase chain reaction; Pathology; Etiology; Heart disease; Molecular biology
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jcp.53.5.382

Aims—Despite the evidence of familial occurrence, chromosomal gene mapping, and apoptosis as a mechanism of myocyte death, the aetiopathogenesis of arrhythmogenic right ventricular cardiomyopathy (ARVC) remains speculative. Because of the frequent histological finding of focal inflammatory infiltrates, the hypothesis of an infective myocarditis aetiology has been put forward. The aim of this investigation was to test this hypothesis. The presence of enteroviruses was investigated by a highly sensitive and specific molecular technique. Methods—Endomyocardial tissue samples from 20 patients with ARVC (11 male, nine female; mean age, 40 years; SD, 16) and 20 control subjects with other cardiac diseases were analysed using reverse transcription and nested polymerase chain reaction (PCR). Myocardial samples obtained from four patients with enteroviral myocarditis and coxsackie B3 virus infected cells were used as positive controls. Results—Endomyocardial biopsy was diagnostic for ARVC in all patients: myocardial atrophy was seen, with less than 45% residual myocytes. Foci of inflammatory infiltrates were seen in four biopsies, and the cells were identified by immunohistochemistry as mainly T cells. All samples, from both patients with ARVC and subjects with other cardiac diseases, were negative for enteroviral genome by means of nested PCR. Conclusion—These findings indicate that enteroviruses are not involved in the aetiopathogenesis of ARVC. Future molecular studies should investigate the presence of other infective agents, as well as their possible role in triggering apoptosis.