Cytotoxic T cells in AIDS colonic cryptosporidiosis

• Published in: Journal of clinical pathology Vol. 54; no. 4; pp. 298 - 303
• Main Authors: Reijasse, D, de Serre, N Patey-Mariaud, Canioni, D, Huerre, M, Haddad, E, Leborgne, M, Blanche, S, Brousse, N
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.04.2001; BMJ; BMJ Publishing Group LTD
• Subjects: acquired immunodeficiency syndrome; Adolescent; Adult; AIDS-Related Opportunistic Infections - immunology; AIDS-Related Opportunistic Infections - parasitology; Apoptosis; Biological and medical sciences; Case-Control Studies; CD8-Positive T-Lymphocytes - immunology; Child; colon; Colon - immunology; Colon - parasitology; cryptosporidiosis; Cryptosporidiosis - immunology; cytotoxicity; Female; Granzymes; Histocytochemistry; Human viral diseases; Humans; immunochemistry; Infectious diseases; Male; Medical sciences; Retrospective Studies; Serine Endopeptidases - metabolism; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Human; Immunohistochemistry; Immunopathology; Effectory cell; Protozoal disease; Pathogenesis; Cytotoxicity; AIDS; Parasitosis; Immune deficiency; Infection; Cryptosporidiosis; Pathology; Viral disease; T-Lymphocyte; Digestive diseases; Intestinal disease; Colitis; Apoptosis
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jcp.54.4.298

Background/Aims—It is not known how enteric cryptosporidiosis induces severe intestinal impairment despite minimal invasion by the parasite. The aim of this study was to analyse the histological features and locally implicated immune cells in colonic biopsies of AIDS related cryptosporidiosis. Patients/Methods—Colonic biopsies from patients with AIDS related cryptosporidiosis (n = 10, group I), patients with AIDS but without intestinal infection (n = 9, group II), and human seronegative controls (n = 9, group III) were studied. Using immunohistochemistry the infiltrating mononuclear cells were analysed in both the epithelium and lamina propria for the expression of CD3, CD8, TiA1, granzyme B, and CD68 and for glandular expression of human major histocompatibility complex DR antigen (HLA-DR). Results—Severe histological changes, resulting in abundant crypt epithelial apoptosis and inflammatory infiltrate in the lamina propria, were seen in all biopsies from group I. A significant increase of CD8+, TiA1+, and granzyme B+ T cells in the lamina propria and HLA-DR glandular expression was noted in group I compared with groups II and III. However, the number of intraepithelial lymphocytes, lamina propria CD3+ T cells, and macrophages was not significantly increased in cryptosporidiosis specimens compared with controls. Conclusion—Epithelial apoptosis mediated by granzyme B+ cytotoxic host T cells might play a major role in the development of colonic lesions in AIDS related cryptosporidiosis.