"Pseudoclusters" and typing by random amplification of polymorphic DNA of Aspergillus fumigatus
This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus usin...
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Published in | Journal of clinical pathology Vol. 48; no. 2; pp. 183 - 184 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
London
BMJ Publishing Group Ltd and Association of Clinical Pathologists
01.02.1995
BMJ BMJ Publishing Group LTD |
Subjects | |
Online Access | Get full text |
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Summary: | This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus using two different preparations of Taq DNA polymerase. One of the enzymes did not discriminate between the isolates, giving the false impression that a cluster of infection had occurred. Enzyme source is thus a key variable when using RAPD to distinguish between isolates of A fumigatus. |
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Bibliography: | PMID:7538151 istex:3A45E55A1D72D9EE857E4A0E6C63C87C66D094C5 local:jclinpath;48/2/183 ark:/67375/NVC-TX7480H7-F href:jclinpath-48-183.pdf ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9746 1472-4146 |
DOI: | 10.1136/jcp.48.2.183 |