Restriction endonuclease analysis of Aspergillus fumigatus DNA

• Published in: Journal of clinical pathology Vol. 45; no. 4; pp. 324 - 327
• Main Authors: Burnie, J P, Coke, A, Matthews, R C
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.04.1992; BMJ; BMJ Publishing Group LTD
• Subjects: Aspergillosis - microbiology; Aspergillus fumigatus - classification; Aspergillus fumigatus - genetics; Biological and medical sciences; DNA Fingerprinting; DNA, Fungal - analysis; Electrophoresis, Agar Gel; Genome, Fungal; Humans; Infectious diseases; Medical sciences; Polymorphism, Restriction Fragment Length; Fungi; Infection; Human; Mycosis; Enzyme; DNA; Fungi Imperfecti; Aspergillus fumigatus; Genome; Thallophyta
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jcp.45.4.324

AIMS: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I. RESULTS: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types. CONCLUSIONS: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.